Black Phosphorus Cytotoxicity Assessments Pitfalls: Advantages and Disadvantages of Metabolic and Morphological Assays
Black Phosphorus Cytotoxicity Assessments Pitfalls: Advantages and Disadvantages of Metabolic and Morphological Assays
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DOI：
10.1002/chem.201804434
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发表时间：
2019-01-02
影响因子：
4.3
通讯作者：
Pumera, Martin
中科院分区：
文献类型：
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作者：
Fojtu, Michaela;Balvan, Jan;Pumera, Martin
黑磷(BP)是一类二维纳米材料，近年来受到越来越多的关注。在BP纳米材料应用日益广泛的同时，对彻底了解其对人类和动物健康的潜在影响的要求也在增加。本研究的目的是比较和讨论五种常用的纳米材料细胞毒性评估方法，特别是BP纳米粒子。对各种因素和陷阱进行了全面调查，在评估其毒性时应考虑这些因素和陷阱，并指出其不一致之处。BP可能会在毒性评估过程中引入不同程度的干扰，具体取决于其应用的浓度。更重要的是，BP的毒性评估被发现受到所选试验性质的影响。它们基于不同的原则，并不需要平等地评估所有的细胞事件。一种基于蛋白酶活性测量的商业试验被确定为最适合BP毒性评估的方法。此外，还强调了时间推移定量相位成像在纳米材料毒性评估中的好处。与传统的评估不同，它提供了对BP管理过程的实时分析，并使其能够在背景下更深入地了解这些过程。
Black phosphorus (BP) belongs to a group of 2D nanomaterials and nowadays attracts constantly increasing attention. Parallel to the growing utilization of BP nanomaterial increase also the requirements for the thorough comprehension of its potential impact on human and animal health. The aim of this study was to compare and discuss five assays commonly used for the cytotoxicity assessments of nanomaterials with a special focus on BP nanoparticles. A comprehensive survey of factors and pitfalls is provided that should be accounted for when assessing their toxicity and pointed to their inconsistency. BP might introduce various levels of interference during toxicity assessments depending on its concentration applied. More importantly, the BP toxicity evaluation was found to be influenced by the nature of assay chosen. These are based on different principles and do not have to assess all the cellular events equally. A commercial assay based on the measurement of protease activity was identified to be the most suitable for the BP toxicity assessment. Further, the benefit of time-lapse quantitative phase imaging for nanomaterial toxicity evaluation was highlighted. Unlike the conventional assessments it provides real-time analysis of the processes accompanying BP administration and enables to understand them deeper and in the context.