Lesional accumulation of heme oxygenase-1 + microglia/macrophages in rat traumatic brain injury
Lesional accumulation of heme oxygenase-1 + microglia/macrophages in rat traumatic brain injury
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DOI：
10.1097/wnr.0b013e32835f2810
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发表时间：
2013-04-17
期刊：
影响因子：
1.7
通讯作者：
Zhang, Zhiren
中科院分区：
文献类型：
--
作者：
Liu, Yuqi;Zhang, Zhiyuan;Zhang, Zhiren
血红素加氧酶-1（HO-1）是血红素降解的诱导限速酶。在这里，我们研究了HO-1的表达在一个开放的头骨重量下降引起的创伤性脑损伤，重点是早期阶段，最适合治疗。在我们研究的正常大鼠脑中，很少观察到HO-1（+）细胞。脑损伤后18 h脑实质内可见HO-1（+）非神经元细胞大量聚集，并持续增加。HO-1（+）非神经元细胞主要分布于病灶周围，表现为活化的小胶质细胞/巨噬细胞表型，形态学特征为分枝状或变形虫状。进一步的双标记实验显示，大部分HO-1（+）非神经元细胞共表达CD 68和CD 163，但不表达胶质细胞酸性蛋白。我们的数据表明，HO-1的表达定义了一个亚型的活化小胶质细胞/巨噬细胞参与创伤性脑损伤后的早期过程。NeuroReport 24：281-286（C）2013年威科健康垂直酒吧Lippincott威廉姆斯&威尔金斯。
Heme oxygenase-1 (HO-1) is an inducible rate-limiting enzyme for heme degradation. Here, we studied the HO-1 expression in an open-skull weight-drop-induced traumatic brain injury, with a focus on the early phase, most amenable to therapy. In normal rat brains of our study, HO-1 (+) cells were rarely observed. Significant parenchymal accumulation of HO-1 (+) non-neuron cells was observed 18 h post-traumatic brain injury and increased continuously during the investigating time. We also observed that the accumulated HO-1 (+) non-neuron cells were mainly distributed in the perilesional areas and showed activated microglia/macrophage phenotypes with ramified or amoeboid morphologic characteristics. Further double-labeling experiments showed that most HO-1 (+) non-neuron cells coexpressed CD68 and CD163, but not glial fibrillary acid protein. Our data suggest that HO-1 expression defines a subtype of activated microglia/macrophages involved in the early processes following traumatic brain injury. NeuroReport 24:281-286 (C) 2013 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.