Population imaging of ongoing neuronal activity in the visual cortex of awake rats
Population imaging of ongoing neuronal activity in the visual cortex of awake rats
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DOI：
10.1038/nn.2140
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发表时间：
2008-07-01
影响因子：
25
通讯作者：
Kerr, Jason N. D.
中科院分区：
文献类型：
--
作者：
Greenberg, David S.;Houweling, Arthur R.;Kerr, Jason N. D.
目前尚不清楚在麻醉动物中记录的正在进行的皮层神经元活动的复杂时空组织与清醒动物的关系。因此，我们在清醒和随后麻醉的大鼠中使用双光子群体钙成像来跟踪不同大脑状态下神经元群体的动作电位放电，并研究单个神经元如何影响群体活动。与麻醉的大鼠相比，清醒的大鼠的放电率和尖峰爆裂率更高，成对相关性更低。麻醉调节了全人群的同步性以及放电率与相关的关系。总的来说，清醒时的大脑活动不能用麻醉来推断。
It is unclear how the complex spatiotemporal organization of ongoing cortical neuronal activity recorded in anesthetized animals relates to the awake animal. We therefore used two-photon population calcium imaging in awake and subsequently anesthetized rats to follow action potential firing in populations of neurons across brain states, and examined how single neurons contributed to population activity. Firing rates and spike bursting in awake rats were higher, and pair-wise correlations were lower, compared with anesthetized rats. Anesthesia modulated population-wide synchronization and the relationship between firing rate and correlation. Overall, brain activity during wakefulness cannot be inferred using anesthesia.