Sequencing and characterization of glycogen synthase and glycogen phosphorylase genes from Spodoptera exigua and analysis of their function in starvation and excessive sugar intake.
Sequencing and characterization of glycogen synthase and glycogen phosphorylase genes from Spodoptera exigua and analysis of their function in starvation and excessive sugar intake.
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DOI：
10.1002/arch.21027
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发表时间：
2012-06
影响因子：
2.2
通讯作者：
B. Tang;Qi Xu;Q. Zou;Qi Fang;Shigui Wang;G. Yè
中科院分区：
文献类型：
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作者：
B. Tang;Qi Xu;Q. Zou;Qi Fang;Shigui Wang;G. Yè
糖原和海藻糖是细菌、真菌、昆虫等生物体发育过程中重要的能量来源和能量代谢的关键调控因子。为了研究甜菜夜蛾糖原代谢途径，首次从甜菜夜蛾中克隆了糖原合成酶（SpoexGS）和糖原磷酸化酶（SpoexGP）的cDNA。exigua. SpoexGS cDNA含有2，010个核苷酸的开放阅读框架，编码669个氨基酸的蛋白质，预测分子量为76.19 kDa，pI为5.84。SpoexGP含有2，946个核苷酸的开放阅读框架，其编码841个氨基酸的蛋白质，预测分子量约为96.63 kDa，pI为6.03。北方杂交结果表明，SpoexGS和SpoexGP基因在猪的脑、脂肪体、中肠、马氏管、精巢和气管中均有表达。exigua. SpoexGS和SpoexGP mRNA在脂肪体中的表达模式相似，但在不同发育阶段的整个身体中的表达模式不同。最后，在饥饿条件下，SpoexGS和SpoexGP的转录表达迅速下降，随着饥饿时间的增加。当饥饿胁迫解除后，SpoexGS和SpoexGP mRNA水平在饥饿6和12 h组低于饥饿24 h组和对照组。与对照组相比，过量糖摄入的治疗导致12小时后SpoexGS和SpoexGP转录物的水平更高。这些发现为糖原合成酶和糖原磷酸化酶蛋白的组织分布、表达模式和潜在功能提供了新的数据。
Glycogen and trehalose are important energy source and key regulation factors in the development of many organisms' pass through energy metabolism, including bacteria, fungi, and insects. To study glycogen metabolism pathway in Spodoptera exigua, first cDNAs for glycogen synthase (SpoexGS) and glycogen phosphorylase (SpoexGP) were cloned from S. exigua. SpoexGS cDNA contains an open reading frame of 2,010 nucleotides encoding a protein of 669 amino acids with a predicted molecular mass of 76.19 kDa and a pI of 5.84. SpoexGP contains an open reading frame of 2,946 nucleotides, which encodes a protein of 841 amino acids with a predicted molecular mass of approximately 96.63 kDa and a pI of 6.03. Second, Northern blotting revealed that SpoexGS and SpoexGP mRNAs were expressed in brain, fat body, mid-gut, Malpighian tubules, spermary, and tracheae of S. exigua. Expression patterns for SpoexGS and SpoexGP mRNAs were similar in fat body, but differed in whole body at different developmental stages. The last, under starvation conditions, SpoexGS and SpoexGP transcript expression rapidly decreased with increasing starvation time. When the starvation stress was removed, SpoexGS and SpoexGP mRNA levels were lower in the groups starved for 6 and 12 h than in the 24-h starvation and control groups. Treatment with excessive sugar intake led to higher levels of SpoexGS and SpoexGP transcripts after 12 h compared to the control group. These findings provide new data on the tissue distribution, expression patterns, and potential function of glycogen synthase and glycogen phosphorylase proteins.