PREVENTION OF ATROPHIC RHINITIS IN PIGLETS BY MEANS OF INTRANASAL ADMINISTRATION OF A LIVE NON-AR-PATHOGENIC BORDETELLA-BRONCHISEPTICA VACCINE
PREVENTION OF ATROPHIC RHINITIS IN PIGLETS BY MEANS OF INTRANASAL ADMINISTRATION OF A LIVE NON-AR-PATHOGENIC BORDETELLA-BRONCHISEPTICA VACCINE
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DOI：
10.1080/01652176.1987.9694089
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发表时间：
1987-04-01
影响因子：
6.4
通讯作者：
DEJONG, MF
中科院分区：
文献类型：
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作者：
DEJONG, MF
在初步研究和田间试验中，研究了仔猪鼻内接种非AR致病性支气管败血波氏杆菌活菌（BB-）对萎缩性鼻炎（AR）的影响。在初步调查中，接种了2日龄SPF小猪（n= 13）。接种后3周，通过AR致病性B（BB+）或AR致病性多杀性巴氏杆菌（PM+）肉汤培养物喷雾进行攻毒。四周后，对仔猪进行尸检，并检查腹侧耳甲（AVC）萎缩。与未接种疫苗的SPF仔猪相比，在BB+和PM+攻毒后，接种疫苗的仔猪显示出强烈且显著的AVC降低。在田间试验中，通过抽签形成三个组：10窝（82头仔猪）接种疫苗; 10窝（92头仔猪）形成对照组，11窝（104头仔猪）接受安慰剂处理。将窝仔分布在两个单元中。在单元1中，AR和PM+被偶然地证明，然而，在单元2中，持续地。在两个单位中，BB+的分离频率相同。对3、6和8周龄仔猪进行临床和细菌学检查。对通过抽签随机选择的41头8周龄仔猪进行尸检。尽管在3周龄时进行了第二次接种，但BB-未充分建立;这可能是由母体BB抗体引起的。在对照组和安慰剂组中，PM+比BB+更早且更频繁地分离。在田间试验中，AVC与PM+的相关性似乎比与BB+的相关性更强。8周龄时患有上短颌症（BS）的仔猪百分比表明了猪群中的AR情况。尽管在单元2中确定了AVC的显著降低，但这不足以表明这种鼻内接种方法在实践中可用于预防AR。
The effect of intranasal vaccination of piglets with live non‐AR‐pathogenicBordetella bronchiseptica(BB‐) against Atrophic Rhinitis (AR) was investigated in a preliminary investigation and in a field trial. In the preliminary investigation 2‐day‐old SPF piglets (n= 13) were vaccinated. Three weeks after vaccination, challenges were carried out by means of a spray with an AR‐pathogenic B (BB+) or an AR‐pathogenic Pasteurella multocida (PM+) broth‐culture. Four weeks later the piglets were necropsied and examined for atrophy of the ventral conchae (AVC). In contrast with the non‐vaccinated SPF piglets, the vaccinated piglets showed a strong and significant reduction of AVC, after both BB+ and PM+ challenge.In the field trial three groups were formed by drawing lots: ten litters (82 piglets) were vaccinated; ten litters (92 piglets) formed the control group and 11 litters (104 piglets) were treated with a placebo. The litters were spread over two units. In unit 1 AR and PM+ were demonstrated incidentally, in unit 2, however, persistently. BB+ was isolated equally frequently in both units.Clinical and bacteriological examinations were done in piglets of 3, 6 and 8 weeks of age.Necropsy examinations was carried out in 41 piglets of 8 weeks of age, chosen randomly by drawing lots.In spite of a second vaccination at the age of 3 weeks, BB‐ was not well established; this was possibly caused by maternal BB antibodies. In the control and placebo groups PM+ was isolated earlier and more frequently than BB+.It appeared that AVC was correlated more strongly with PM+ than with BB+ infection in the field trial. The percentage of piglets with Brachygnathia superior (BS) at the age of 8 weeks indicated the AR situation in the herd.Although a significant reduction of AVC was determined in unit 2, it was not sufficient to indicate that this method of intranasal vaccination is useful in the prevention of AR in practice.