Alterations in peripheral B cells and B cell progenitors following androgen ablation in mice
Alterations in peripheral B cells and B cell progenitors following androgen ablation in mice
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DOI：
10.1093/intimm/13.4.553
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发表时间：
2001-04-01
影响因子：
4.4
通讯作者：
Kwon, ED
中科院分区：
文献类型：
--
作者：
Ellis, TM;Moser, MT;Kwon, ED
B淋巴细胞的产生部分地受雄激素和雌激素的生理水平调节。虽然这些性激素下调B淋巴细胞生成，但在雄激素或雌激素水平降低的条件下发生B淋巴细胞生成的增加。在这项研究中，我们检查雄激素消融雄性小鼠的B淋巴细胞生成和外周B淋巴细胞群体的表型组成的影响。脾脏和胸腺重量在去势后显著增加，外周血淋巴细胞总数也是如此。然而，在去势后，外周中a细胞的绝对数量选择性地增加; T细胞、NK细胞和粒细胞的数量保持不变。循环B细胞的增加主要是由于表达B220（Io+）CD 24（hi+）表型的近期骨髓移行细胞数量的增加，并且这些细胞在去势小鼠中保持显著升高，直至去势后54天。在缺乏功能性雄激素受体（Tfm）的小鼠中观察到新迁移的B细胞的百分比的类似增加。最后，骨髓中B细胞祖细胞的评估显示IL-7应答性B细胞祖细胞的相对数量显著增加，包括哈代部分B（早期前B细胞）、C（晚期前B细胞）、D（前B细胞）和E（未成熟B细胞）中的细胞。这些发现表明，阉割后的雄激素去除显着且选择性地改变了小鼠外周B细胞的组成。此外，这些改变是由于雄激素消融对IL-7应答性前B细胞祖细胞的增强作用所致。
The production of B lymphocytes is regulated in part by physiologic levels of androgens and estrogens, While these sex hormones down-regulate B lymphopoiesis, augmentation of B lymphopoiesis occurs under conditions where androgen or estrogen levels are decreased. In this study we examine the effect of androgen ablation of male mice on B lymphopoiesis and on the phenotypic composition of peripheral B lymphocyte populations. Spleen and thymic weights are significantly increased following castration, as is the total number of peripheral blood lymphocytes. However, the absolute numbers of a cells in the periphery are selectively increased following castration; the numbers of T cells, NK cells and granulocytes remain unchanged. The increase in circulating B cells is due largely to increases in the numbers of recent bone marrow emigrants expressing a B220(Io+) CD24(hi+) phenotype and these cells remain significantly elevated in castrated mice for up to 54 days post-castration. Similar increases in the percentages of newly emigrated B cells are observed in mice that lack a functional androgen receptor (Tfm), Finally, assessments of B cell progenitors in the bone marrow revealed significant increases in the relative numbers of IL-7-responsive B cell progenitors, including cells in Hardy fractions B (early pro-B cells), C (late pro-B cells), D (pre-B cells) and E (immature B cells). These findings demonstrate that androgen ablation following castration significantly and selectively alters the composition of peripheral B cells in mice. Further, these alterations result from the potentiating effects of androgen ablation on IL-7-responsive pro-B cell progenitors.