Vitamin E reduces endosulfan-induced toxic effects on morphology and behavior in early development of zebrafish (Danio rerio)
Vitamin E reduces endosulfan-induced toxic effects on morphology and behavior in early development of zebrafish (Danio rerio)
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DOI：
10.1016/j.fct.2017.01.004
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发表时间：
2017-03-01
影响因子：
4.3
通讯作者：
Ellingsen, S.
中科院分区：
文献类型：
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作者：
Dale, K.;Rasinger, J. D.;Ellingsen, S.
本研究的目的是使用改良的斑马鱼胚胎毒性试验（ZET）调查维生素E（α-TOC）是否调节农药硫丹（ESF）的发育毒性。斑马鱼（Danio rerio）胚胎暴露于ESF（0.1-50 mg/L）或α-TOC（0.01 - 3 mM）单独或组合6至72 h后受精（hpf）。分析了这些暴露对胚胎形态、幼虫行为和抗氧化基因表达的影响。在48 hpf的表型分析表明，ESF导致胚胎畸形的剂量依赖性增加，包括轴畸形，心包水肿和色素沉着减少。ESF与α-TOC（1-3 mM）的共暴露显著（p < 0.05）减少了ESF诱导的胚胎畸形。仅接触α-TOC不会影响存活率或畸形率。行为学研究表明，ESF在受精后5天引起多动，表明发育神经毒性作用。ESF诱导的多动被α-TOC改善。升高的ESF浓度引起抗氧化基因cuzn-sod，gpxl a和cat的下调，表明ESF促进了胚胎中的氧化应激。α-TOC不能阻止ESF诱导的这些基因的失调。这些结果表明，α-TOC保护对表型和行为的影响所造成的ESF，但没有拯救ESF诱导的抗氧化基因表达的畸变。(C)2017爱思唯尔有限公司版权所有
The aim of this study was to investigate if vitamin E (alpha-TOC) modulates the developmental toxicity of the pesticide endosulfan (ESF), using a modified zebrafish embryotoxicity test (ZET). Zebrafish (Danio rerio) embryos were exposed from 6 to 72 h post fertilization (hpf) to either ESF (0.1-50 mg/L) or alpha-TOC (0.01 3 mM) alone or in combination. The effects of these exposures on embryonic morphology, larval behavior and antioxidant gene expression were analyzed. Phenotypic analysis at 48 hpf showed that ESF led to a dose-dependent increase in embryonic deformities, including axis malformations, pericardial edema and reduced pigmentation. Co-exposure of ESF with a-TOC (1-3 mM) significantly (p < 0.05) reduced ESF-induced embryonic malformations. Exposure to solely alpha-TOC did not affect rates of survival or malformations. Behavior studies showed that ESF caused hyperactivity at 5 days post fertilization, indicating a developmental neurotoxic effect. The ESF-induced hyperactivity was ameliorated by alpha-TOC. Elevated ESF concentrations caused down-regulation of the antioxidant genes cuzn-sod, gpxl a and cat, suggesting that ESF promoted oxidative stress in the embryos. alpha-TOC did not prevent the ESF-induced dysregulation of these genes. These results demonstrate that alpha-TOC protects against phenotypic and behavioral effects caused by ESF but did not rescue ESF-induced aberrations in antioxidant gene expression.(C) 2017 Elsevier Ltd. All rights reserved.