Clinical outcome and vertical transmission variability among canine Neospora caninum isolates in a pregnant mouse model of infection
Clinical outcome and vertical transmission variability among canine Neospora caninum isolates in a pregnant mouse model of infection
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DOI：
10.1017/s0031182013001479
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发表时间：
2014-03-01
期刊：
影响因子：
2.4
通讯作者：
Ortega-Mora, Luis M.
中科院分区：
文献类型：
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作者：
Dellarupe, Andrea;Regidor-Cerrillo, Javier;Ortega-Mora, Luis M.
我们使用妊娠BALB/c模型比较了6种犬新孢子虫分离株的临床结局和垂直传播。其中4个分离株来自自然感染犬的卵囊（Nc-Ger 2、Nc-Ger 3、Nc-Ger 6和Nc-6Arg），2个分离株来自具有神经系统体征的患病犬（Nc-Bahia和Nc-Liv）。在妊娠第7天，用每种分离株的2 × 106个速殖子接种母鼠。在母鼠和后代中评价发病率、死亡率和抗体应答，以及寄生虫向后代的传播。死亡率从Nc-Bahia和Nc-Liv感染的幼崽的100%到感染卵囊分离株的幼崽的19%或更低不等。垂直传播率为9 ~ 53%。犬卵囊，相比之下，100%的Nc-Liv和Nc-Bahia分离株。所有母鼠均显示出针对速殖子和rNc-GRA 7抗原的特异性IgG应答，证实了新孢子虫感染。在接种Nc-Liv和Nc-Bahia分离株的小鼠中检测到最高IgG水平。这些结果表明，N.从卵囊和神经学上受影响的狗获得的犬分离物。这种变异性可以帮助我们解释最终宿主和中间宿主感染结果的差异。
We compared the clinical outcome and vertical transmission of six canine Neospora caninum isolates using a pregnant BALB/c model. Four of the isolates were obtained from oocysts of naturally infected dogs (Nc-Ger2, Nc-Ger3, Nc-Ger6 and Nc-6Arg) and two were from diseased dogs with neurological signs (Nc-Bahia and Nc-Liv). The dams were inoculated with 2x10(6) tachyzoites of each isolate at day 7 of pregnancy. Morbidity, mortality and the antibody responses were evaluated in both the dams and the offspring, as was parasite transmission to the progeny. The mortality rates varied from 100% in Nc-Bahia and Nc-Liv-infected pups to 19% or less for those infected with the isolates from oocysts. The vertical transmission rates varied from 9 to 53% for N. caninum from oocysts, compared with 100% for the Nc-Liv and Nc-Bahia isolates. All dams showed specific IgG responses against tachyzoite and rNc-GRA7 antigens, confirming Neospora infection. The highest IgG levels were detected in mice inoculated with the Nc-Liv and Nc-Bahia isolates. These results demonstrate marked differences in virulence between the N. caninum isolates obtained from oocysts and neurologically affected dogs. This variability could help us to explain the differences in the outcome of the infection in definitive and intermediate hosts.