Stable isotope probing of bacterial community structure and gene expression in the rhizosphere of Arabidopsis thaliana
Stable isotope probing of bacterial community structure and gene expression in the rhizosphere of Arabidopsis thaliana
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DOI：
10.1111/j.1574-6941.2012.01345.x
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发表时间：
2012-08-01
影响因子：
4.2
通讯作者：
Achouak, Wafa
中科院分区：
文献类型：
--
作者：
Haichar, Feth el Zahar;Roncato, Marie-Anne;Achouak, Wafa
根际是植物和微生物建立分子对话的活跃空间。在本研究中，我们利用DNA-和mRNA-SIP分析了在13CO2条件下生长13、21和27天的拟南芥根际细菌群落结构和某些有益基因表达的影响。DNA-和rRNA-SIP揭示了根际土壤细菌群落的变化，这些变化可能与根分泌物的修饰有关，而根定殖种群则随着时间的推移而保持不变，这表明它们的代谢多样性和适应性。利用mRNA-SIP检测了植物通过根系分泌物对根附着土壤中编码1-氨基环丙烷-1-羧酸脱氨酶的acdS基因rsmZ和编码氧化亚氮还原酶的nosZ基因表达的影响。结果表明，这些基因存在于栖息在根部的细菌中，也存在于从土壤有机物分解或根部分泌物中获取养分的细菌中。rsmZ在自然条件下的表达表明非编码rna在细菌适应生态位中的重要性。
The rhizosphere is an active compartment where plant and microorganisms establish a molecular dialogue. In this study, we analysed the impact of Arabidopsis thaliana on bacterial community structure and the expression of certain beneficial genes using DNA- and mRNA-SIP in the rhizosphere of plantlets grown under 13CO2 for 13, 21 and 27 similar to days. DNA- and rRNA-SIP revealed changes in bacterial communities inhabiting the rhizosphere soil that were probably related to modification of root exudates, while root-colonizing populations were maintained over time suggesting their metabolic versatility and adaptation. The impact of the plant via root exudates on the expression of the noncoding RNAs rsmZ, acdS gene encoding 1-aminocyclopropane-1-carboxylate deaminase and nosZ gene encoding nitrous oxide reductase, in the root-adhering soil and on the roots of A.similar to thaliana was determined using mRNA-SIP. Results showed that these genes were present and expressed by bacteria inhabiting roots and by those that derive nutrients from the breakdown of organic matter in soils or from root exudates. The expression of rsmZ under natural conditions indicates the importance of noncoding RNAs in bacterial adaptation to their ecological niches.