Interaction of leptospires with murine microglial cells.
Interaction of leptospires with murine microglial cells.
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钩端螺旋体与小鼠小胶质细胞的相互作用。
DOI：
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发表时间：
2006
期刊：
影响因子：
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通讯作者：
E. Blasi
中科院分区：
文献类型：
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作者：
M. Cinco;R. Domenis;S. Perticarari;G. Presani;A. Marangoni;E. Blasi
钩端螺旋体是钩端螺旋体病的病原体，在哺乳动物感染过程中对中枢神经系统产生趋向性。我们主要通过流式细胞术分析评估了小鼠小胶质细胞与致病性问号钩端螺旋体和非致病性双折钩端螺旋体菌株之间的相互作用。在缺乏调理条件的情况下，小胶质细胞能够吞噬——甚至相当缓慢——螺旋体并杀死非致病菌株。对小胶质细胞的粘附是快速且与所有菌株相关的，不涉及 CR3 整联蛋白受体。这些发现表明，在体外非调理条件下，小鼠小胶质细胞不能有效清除致病性钩端螺旋体。
Leptospires, the agents of leptospirosis, exert tropism for the central nervous system, in the course of mammal infection. We evaluated the interaction between murine microglial cells and strains of pathogenic L. interrogans leptospires and non-pathogenic L. biflexa leptospires, mainly by flow cytometric assays. In the absence of opsonic conditions microglia are capable of ingesting--even quite slowly--the spirochetes and killing the non-pathogenic strain. The adhesion to microglia, which is quick and relevant for all the strains, does not involve the CR3 integrin receptor. These findings suggest that the murine microglia--in non opsonic conditions in vitro--do not effectively clear the pathogenic leptospires.