Comparison of real-time PCR detection methods for B1 and P30 genes of Toxoplasma gondii
Comparison of real-time PCR detection methods for B1 and P30 genes of Toxoplasma gondii
复制标题
DOI：
10.1016/s0732-8893(02)00549-7
复制
发表时间：
2003-04-01
影响因子：
2.9
通讯作者：
Rodloff, AC
中科院分区：
文献类型：
--
作者：
Buchbinder, S;Blatz, R;Rodloff, AC
以弓形虫P30和35拷贝的B1基因为靶基因，建立了实时荧光定量聚合酶链式反应检测方法。两种聚合酶链式反应方法每次检测的弓形虫DNA在10-100000个基因组当量之间，且具有相当的批内偏差，这表明检测单个拷贝位点适合于临床诊断。(C)2003 Elsevier Science Inc.保留所有权利。
Real-time PCR assays were established with the Toxoplasma gondii P30 and the 35-copy B1 gene as target genes. Both PCR methods detected Toxoplasma DNA from 10 to 100000 genome equivalents per assay and had comparable intra-assay deviations suggesting that detecting a single copy locus is suitable for clinical diagnostic. (C) 2003 Elsevier Science Inc. All rights reserved.