Ultrasensitive densitometry detection of cytokines with nanoparticle-modified aptamers
Ultrasensitive densitometry detection of cytokines with nanoparticle-modified aptamers
复制标题
用纳米颗粒修饰的适体进行细胞因子的超灵敏光密度检测
DOI：
10.1373/clinchem.2006.082271
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发表时间：
2007-06-01
影响因子：
9.3
通讯作者：
Xu, Shun-Qing
中科院分区：
文献类型：
--
作者：
Li, Yuan-Yuan;Zhang, Chi;Xu, Shun-Qing
背景：适体模拟抗体的特性，有时甚至比抗体更好地作为测定试剂。我们描述了一个超灵敏的密度法细胞因子检测纳米粒子（NP）-modified aptamers.Methods的建立：该测定同时使用金NP-改性的适配体和生物素-改性的适配体结合到靶蛋白，形成一个三明治复合物。结果：血小板衍生生长因子B链同源二聚体（PDGF-BB）的检测范围为1 fmol/L ~ 100 pmol/L，线性关系良好（R-2 = 0.9869）。分析检测限为83阿莫尔/L。测定内和测定间不精密度（CV）为
Background: Aptamers mimic properties of antibodies and sometimes turn out to be even better than antibodies as reagents for assays. We describe the establishment of an ultrasensitive densitometry method for cytokine detection by nanoparticle (NP)-modified aptamers.Methods: The assay simultaneously uses a gold NP-modified aptamer and a biotin-modified aptamer to bind to the target protein, forming a sandwich complex. The absorbance signal generated by the aptamer-protein complex is amplified and detected with a microplate reader.Results: The assay for platelet-derived growth factor B-chain homodimer (PDGF-BB) was linear from I fmol/L to 100 pmol/L (R-2 = 0.9869). The analytical detection limit was 83 amol/L. The intraassay and interassay imprecision (CVs) was