Cholesterol-Phospholipid Interactions: New Insights from Surface X-Ray Scattering Data
Cholesterol-Phospholipid Interactions: New Insights from Surface X-Ray Scattering Data
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DOI：
10.1103/physrevlett.104.108101
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发表时间：
2010-03-12
影响因子：
8.6
通讯作者：
Gidalevitz, David
中科院分区：
文献类型：
--
作者：
Ivankin, Andrey;Kuzmenko, Ivan;Gidalevitz, David
我们报道了用X射线反射率和掠入射X射线衍射法研究胆固醇-DPPC(1，2-二棕榈酰基-3-甘油-3-磷胆碱)单分子膜的结构。反射率表明，胆固醇相对于磷脂的垂直位置强烈依赖于其摩尔分数(chi(Chol))。此外，我们还发现在较宽的CHI(CHOL)范围内，胆固醇和DPPC形成了与薄膜相同的化学计量比的短程有序的合金化混合微区。在现有数据的基础上，我们提出了混合单层中胆固醇-磷脂组织的新模型。
We report a structural study of cholesterol-DPPC (1,2-dipalmitoyl-sn-glycero-3-phophocholine) monolayers using x-ray reflectivity and grazing incidence x-ray diffraction. Reflectivity reveals that the vertical position of cholesterol relative to phospholipids strongly depends on its mole fraction (chi(CHOL)). Moreover, we find that at a broad range of chi(CHOL) cholesterol and DPPC form alloylike mixed domains of short-range order and the same stoichiometry as that of the film. Based on the data presented, we propose a new model of cholesterol-phospholipid organization in mixed monolayers.