Relationship between seizure frequency and number of neuronal and non-neuronal cells in the hippocampus throughout the life of rats with epilepsy
Relationship between seizure frequency and number of neuronal and non-neuronal cells in the hippocampus throughout the life of rats with epilepsy
复制标题
DOI：
10.1016/j.brainres.2015.12.055
复制
发表时间：
2016-03-01
期刊：
影响因子：
2.9
通讯作者：
Arida, Ricardo Mario
中科院分区：
文献类型：
--
作者：
Lopim, Glauber Menezes;Campos, Diego Vannucci;Arida, Ricardo Mario
癫痫发作频率和细胞死亡之间的关系一直是一个有争议的话题。为了解决这个问题，我们使用匹鲁卡品模型测定了癫痫大鼠一生中癫痫发作的频率和海马细胞的总数。癫痫动物的癫痫发作频率因其所处的生命期而异，癫痫发作次数逐渐增加，直到癫痫发作生命的180天（6个月），然后减少（330天-11个月），随后癫痫发作稳定。通过各向同性分离的细胞计数显示，与对照组相比，大鼠自发性复发性癫痫发作（SRS）30、90、180和360天后海马神经元细胞数量减少（约25%-30%的神经元细胞减少）。此外，与癫痫发作90天和180天的动物相比，SRS发作360天的动物显示神经元细胞数量减少。癫痫大鼠海马非神经元细胞总数在SRS后30天明显减少，但在第90、180和360天无明显变化。神经元细胞总数与癫痫发作频率呈负相关，表明一生中癫痫发作的发生与神经元丢失之间存在关联。总之，我们的研究结果增加了新的数据有关的时间过程中的SRS和海马细胞数量在整个癫痫生活的文献。(C)© 2016 Elsevier B. V.版权所有。
The relationship between seizure frequency and cell death has been a subject of controversy. To tackle this issue, we determined the frequency of seizures and the total number of hippocampal cells throughout the life of rats with epilepsy using the pilocarpine model. Seizure frequency varied in animals with epilepsy according to which period of life they were in, with a progressive increase in the number of seizures until 180 days (sixth months) of epileptic life followed by a decrease (330 days-eleventh month) and subsequently stabilization of seizures. Cell counts by means of isotropic fractionation showed a reduction in the number of hippocampal neuronal cells following 30, 90, 180 and 360 days of spontaneous recurrent seizures (SRS) in rats compared to their controls (about 25%-30% of neuronal cell reduction). In addition, animals with 360 days of SRS showed a reduction in the number of neuronal cells when compared with animals with 90 and 180 days of seizures. The total number of hippocampal non-neuronal cells was reduced in rats with epilepsy after 30 days of SRS, but no significant alteration was observed on the 90th, 180th and 360th days. The total number of neuronal cells was negatively correlated with seizure frequency, indicating an association between occurrence of epileptic seizures throughout life and neuronal loss. In sum, our results add novel data to the literature concerning the time-course of SRS and hippocampal cell number throughout epileptic life. (C) 2016 Elsevier B.V. All rights reserved.