Two new members of the OmpR superfamily detected by homology to a sensor-binding core domain.
Two new members of the OmpR superfamily detected by homology to a sensor-binding core domain.
复制标题
通过与传感器结合核心结构域的同源性检测到 OmpR 超家族的两个新成员。
DOI：
10.1007/bf02602935
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发表时间：
1989
影响因子：
3.9
通讯作者：
Moses,RE
中科院分区：
文献类型：
--
作者：
Timme,TL;Lawrence,CB;Moses,RE
OmpR超家族包括作为对环境刺激作出反应的操作子的转录调节因子的蛋白质。n端附近的同源结构域，称为传感器结合核心结构域，被认为在信号转导蛋白的识别中起作用。我们已经确定了该调节蛋白家族的两个先前未被识别的成员：从uvrc转录单元转录的23.8 kd蛋白和PgtA基因产物，这是一种磷酸甘油转运调节蛋白。传感器结合核心结构域也存在于四种调节细菌孢子形成和趋化的蛋白质中。这个23.8 kd的蛋白也与延伸因子Tu和两个调节蛋白具有序列相似性：HtpR（热休克调节蛋白）和TraJ（参与偶联的基因表达调节剂）。23.8-kd蛋白的c端附近有一个77个氨基酸的区域，与uvrc转录单元中23.8-kd蛋白的开放阅读框5′编码的28.1-kd蛋白有30%的相似性。具有传感器结合核心结构域的蛋白的氨基酸序列遗传距离分析表明，23.8 kd蛋白和趋化性调节蛋白与OmpR超家族中的其他调节蛋白具有远亲关系。
The OmpR superfamily includes proteins that act as transcriptional regulators of operons that respond to environmental stimuli. A homologous domain near the N-terminus, termed a sensor-binding core domain, is thought to play a role in recognition of a signal transduction protein. We have identified two previously unrecognized members of this regulator family of proteins: a 23.8-kd protein transcribed from theuvrCtranscription unit and the PgtA gene product, which is a phosphoglycerate transport regulatory protein. The sensor-binding core domain is also present in four proteins that regulate bacterial sporulation and chemotaxis. The 23.8-kd protein also has sequence similarity to elongation factor Tu and two regulatory proteins: HtpR, the heat-shock regulatory protein, and TraJ, a regulator of expression of genes involved in conjugation. There is a 77-amino acid region near the C-terminus of the 23.8-kd protein that has 30% similarity with a 28.1-kd protein coded for by an open reading frame 5′ to the reading frame of the 23.8-kd protein in theuvrCtranscription unit. Genetic distance analysis of amino acid sequences of proteins with a sensor-binding core domain suggests that the 23.8-kd protein and the chemotaxis regulatory proteins are distantly related to the other regulatory proteins in the OmpR superfamily.