CROHNS-DISEASE - TRANSMISSION ELECTRON-MICROSCOPIC STUDIES .2. IMMUNOLOGICAL INFLAMMATORY RESPONSE - ALTERATIONS OF MAST-CELLS, BASOPHILS, EOSINOPHILS, AND THE MICROVASCULATURE
CROHNS-DISEASE - TRANSMISSION ELECTRON-MICROSCOPIC STUDIES .2. IMMUNOLOGICAL INFLAMMATORY RESPONSE - ALTERATIONS OF MAST-CELLS, BASOPHILS, EOSINOPHILS, AND THE MICROVASCULATURE
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DOI：
10.1016/s0046-8177(80)80072-4
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发表时间：
1980-01-01
期刊：
影响因子：
3.3
通讯作者：
DICKERSIN, GR
中科院分区：
文献类型：
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作者：
DVORAK, AM;MONAHAN, RA;DICKERSIN, GR
使用12例克罗恩病患者和3例对照受试者的手术切除标本进行透射电子显微镜检查[TEM]。选择回肠和近端未受累的切除切缘的无结受累区域进行研究。制备用于TEM的样品以通过各种技术进行观察。通过光学显微镜研究了Epon包埋块（605个），并在TEM中观察了其中的112个。肥大细胞的数量显着增加，它们主要被发现在水肿的粘膜下层和平滑肌细胞之间的肌层的参与肠道。肥大细胞的局灶性和完全脱颗粒的证据是常见的。鉴定出嗜碱性白细胞，其中一些已脱颗粒。另一种常见的细胞，嗜酸性粒细胞，显示颗粒的变化，这可能反映了嗜酸性粒细胞颗粒物质的释放。嗜酸性粒细胞含有增加的芳基硫酸酯酶型小致密颗粒。嗜酸性粒细胞、嗜碱性粒细胞和肥大细胞形成密切的联系。存在急性和慢性血管内皮损伤的证据。存在小淋巴细胞、浆细胞、巨噬细胞和上皮样细胞;很少遇到多核巨细胞;很少见到成淋巴细胞。
Transmission electron microscopy [TEM] was done using surgically resected specimens from 12 patients with Crohn''s disease and 3 control subjects. Nonuclerated involved areas of ileum and proximal, grossly uninvolved resection margins were chosen for study. Specimens for TEM were prepared for viewing by a variety of techniques. Epon embedded blocks (605) were studied by light microscopy and 112 of these were viewed in TEM. The number of mast cells was markedly increased, and they were found predominantly in edematous submucosa and between smooth muscle cells in the muscular coats of the involved gut. Evidence of focal and complete degranulation of mast cells was frequently seen. Basophilic leukocytes, some of which had degranulated, were identified. Another frequently encountered cell, the esoinophil, showed changes in the granules, which might reflect the release of eosinophil granule materials. Eosinophils contained an increased number of small dense granules of the arylsulfatase containing type. Eosinophils, basophils and mast cells formed close associations. Evidence of acute and chronic damage to vascular endothelia was present. Small lymphocytes, plasma cells, macrophages and epithelioid cells were present; multinucleated giant cells were infrequently encountered; and lymphoblasts were rarely seen.