Intracellular pH sensing using p-aminothiophenol functionalized gold nanorods with low cytotoxicity.
Intracellular pH sensing using p-aminothiophenol functionalized gold nanorods with low cytotoxicity.
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DOI：
10.1021/ac200467z
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发表时间：
2011-05
影响因子：
7.4
通讯作者：
S. Zong;Zhuyuan Wang;Jing Yang;Yiping Cui
中科院分区：
文献类型：
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作者：
S. Zong;Zhuyuan Wang;Jing Yang;Yiping Cui
报道了一种基于表面增强拉曼散射(SERS)的细胞内pH传感器，该传感器以盐酸(HCl)处理的金纳米棒(GNRs)为底物，对氨基硫酚(PATP)为拉曼报告分子。使用我们之前报道的经盐酸处理的GNRs，其生物兼容性和SERS性能都有了很大的提高。同时，被吸附的记者可以直接暴露在周围环境中，这对于生物传感器来说是非常重要的。结果表明，p-ATP的表面增强拉曼光谱强烈依赖于pH值。PH值在3.0~8.0范围内，1142 cm(-1)、1390 cm(-1)和1432 cm(-1)处的SERS谱带强度明显增大。PATP功能化的盐酸处理的GNRs在将GNRs掺入活的HeLa细胞后，这种pH依赖的SERS性能被很好地保留了下来。我们的实验结果表明，这种pATP功能化的盐酸处理的GNRs可以作为一种有效的细胞内pH传感器。因此，我们展示了一个很好的例子，即经过简单的盐酸处理后，正常的CTAB稳定的GNRs的生物应用可以扩大。
We report an intracellular pH sensor based on surface enhanced Raman scattering (SERS) using the hydrochloric acid (HCl) treated gold nanorods (GNRs) as the SERS substrates and p-aminothiophenol (pATP) as the Raman reporter. Using the HCl treated GNRs previously reported by us, the biocompatibility and the SERS performance of GNRs have been greatly improved. Meanwhile, the adsorbed reporters are allowed to be directly exposed to the surrounding environments, which is very important for biosensors. It is found that the SERS spectrum of pATP is strongly dependent on the pH value. The intensities of SERS bands at 1142 cm(-1), 1390 cm(-1), and 1432 cm(-1) increased obviously with the pH value varying from 3.0 to 8.0. This pH-dependent SERS performance of pATP-functionalized HCl treated GNRs was well retained after the incorporation of the GNRs into living HeLa cells. Our experimental results indicate that such pATP-functionalized HCl treated GNRs can be used as an effective intracellular pH sensor. Thus, we show a good example that the bioapplications of the normal CTAB-stabilized GNRs can be expanded after the simple HCl treatment.