Association of Fusobacterium nucleatum infection with colorectal cancer in Chinese patients
Association of Fusobacterium nucleatum infection with colorectal cancer in Chinese patients
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DOI：
10.3748/wjg.v22.i11.3227
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发表时间：
2016-03-21
影响因子：
4.3
通讯作者：
Nie, Yu-Qiang
中科院分区：
文献类型：
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作者：
Li, Yu-Yuan;Ge, Quan-Xing;Nie, Yu-Qiang
目的：研究具核梭杆菌（Fusobacteriumnucleatum，F.方法：收集101例结直肠癌患者手术切除的癌组织及癌旁正常组织（癌切缘外10 cm）。应用荧光定量聚合酶链反应（PCR）检测F。结直肠癌和正常组织中的核仁。F.癌组织与正常组织核质丰度的关系及F.核丰度与临床变量进行了评价。对22例F值最高的大肠癌组织进行荧光原位杂交（FISH）分析。结果：F.结直肠癌组织[0.242（0.178-0.276）]显著高于正常对照组[0.050（0.023-0.067）]（P < 0.001）。F.在88/101例（87.1%）CRC样本中，有核细胞过度表达。F.肿瘤组织中2-（Delta CT）测定的细胞核密度[0.242（0.178，0.276）]显著高于正常对照组[0.050（0.023，0.067）]（P < 0.001）。高丰度组淋巴结转移率[52/88（59.1%）]高于低丰度组[0/13（0%）]（P < 0.005）。F.与其他临床病理指标比较，P > 0.05。FISH分析也发现了更多的F。结直肠癌组织中有核细胞数中位数为6，25 th 3，75 th 10 vs 2，25 th 1，75 th 5（P < 0.01）。结直肠癌组织中有核质富集，与结直肠癌的发生和转移有关。
AIM: To investigate Fusobacterium nucleatum (F. nucleatum) abundance in colorectal cancer (CRC) tissues and its association with CRC invasiveness in Chinese patients.METHODS: The resected cancer and adjacent normal tissues (10 cm beyond cancer margins) from 101 consecutive patients with CRC were collected. Fluorescent quantitative polymerase chain reaction (FQ-PCR) was applied to detect F. nucleatum in CRC and normal tissues. The difference of F. nucleatum abundance between cancer and normal tissues and the relationship of F. nucleatum abundance with clinical variables were evaluated. Fluorescence in situ hybridization (FISH) analysis was performed on 22 CRC tissues with the highest F. nucleatum abundance by FQ-PCR testing to confirm FQ-PCR results.RESULTS: The median abundance of F. nucleatum in CRC tissues [0.242 (0.178-0.276)] was significantly higher than that in normal controls [0.050 (0.023-0.067)] (P < 0.001). F. nucleatum was over-represented in 88/101 (87.1%) CRC samples. The abundance of F. nucleatum determined by 2-(Delta CT) was significantly greater in tumor samples [0.242 (0.178, 0.276)] than in normal controls [0.050 (0.023, 0.067)] (P < 0.001). The frequency of patients with lymph node metastases was higher in the over-abundance group [52/88 (59.1%)] than in the under-abundance group [0/13 (0%)] (P < 0.005). No significant association of F. nucleatum with other clinico-path-ological variables was observed (P > 0.05). FISH analysis also found more F. nucleatum in CRC than in normal tissues (median number 6, 25th 3, 75th 10 vs 2, 25th 1, 75th 5) (P < 0.01).CONCLUSION: F. nucleatum was enriched in CRC tissues and associated with CRC development and metastasis.