Molecular basis for dimethylsulfoxide (DMSO) action on lipid membranes
Molecular basis for dimethylsulfoxide (DMSO) action on lipid membranes
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DOI：
10.1021/ja063363t
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发表时间：
2006-11-01
影响因子：
15
通讯作者：
Anwar, Jamshed
中科院分区：
文献类型：
--
作者：
Notman, Rebecca;Noro, Massimo;Anwar, Jamshed
二甲基亚砜（DMSO）是一种非质子溶剂，具有诱导细胞融合和细胞分化以及增强脂膜通透性的能力。它也是一种有效的冷冻保护剂。深入了解这种分子如何调节膜结构和功能对于调节上述过程和开发用于增强或阻碍生物活性分子吸收的化学手段，特别是进入或经由皮肤的吸收，将是非常宝贵的。我们在这里表明，通过分子模拟，DMSO可以诱导水孔在二棕榈酰磷脂酰胆碱双层，并提出这是一个可能的途径，通过脂质膜的活性物质的渗透增强。DMSO还导致膜变得更松软，这将增强渗透性，促进膜融合，并使细胞膜能够在冷冻保存期间适应渗透和机械应力。
Dimethylsulfoxide (DMSO) is an aprotic solvent that has the ability to induce cell fusion and cell differentiation and enhance the permeability of lipid membranes. It is also an effective cryoprotectant. Insights into how this molecule modulates membrane structure and function would be invaluable toward regulating the above processes and for developing chemical means for enhancing or hindering the absorption of biologically active molecules, in particular into or via the skin. We show here by means of molecular simulations that DMSO can induce water pores in dipalmitoyl-phosphatidylcholine bilayers and propose this to be a possible pathway for the enhancement of penetration of actives through lipid membranes. DMSO also causes the membrane to become floppier, which would enhance permeability, facilitate membrane fusion, and enable the cell membrane to accommodate osmotic and mechanical stresses during cryopreservation.