The expression of SARS-CoV-2 receptor ACE2 and CD147, and protease TMPRSS2 in human and mouse brain cells and mouse brain tissues
The expression of SARS-CoV-2 receptor ACE2 and CD147, and protease TMPRSS2 in human and mouse brain cells and mouse brain tissues
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SARS-CoV-2受体ACE2和CD147以及蛋白酶TMPRSS2在人和小鼠脑细胞和小鼠脑组织中的表达
DOI：
10.1016/j.bbrc.2020.09.042
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发表时间：
2020-12-17
影响因子：
3.1
通讯作者：
Sun, Binlian
中科院分区：
文献类型：
--
作者：
Qiao, Jialu;Li, Weiling;Sun, Binlian
严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)被发现是引起冠状病毒病19(新冠肺炎)的病原。SARS-CoV-2的细胞侵入是由病毒的刺突糖蛋白、宿主特异性受体和蛋白酶介导的。最近，除了以肺部并发症为主要症状外，调查还显示，SARS-CoV-2可引发神经系统症状。因此，研究新冠肺炎神经病变中受体及相关蛋白的表达水平对了解其神经病变具有重要意义。采用定量逆转录聚合酶链式反应(QRT-PCR)和免疫印迹法检测ACE2受体、CD147受体和丝氨酸蛋白酶TMPRSS2在人脑细胞系和小鼠不同脑区的表达水平。结果表明，它们的表达模式都与肺有很大的不同。与肺细胞系和组织相比，ACE2在大多数脑细胞系和小鼠脑组织中表达较低，而CD147在小鼠脑组织中表达较高，TMPRSS2在脑细胞系和小鼠肺组织中表达一致。提示SARS-CoV-2可能对大脑神经系统有不同的感染途径。我们的发现将为预测SARS-CoV-2感染对人脑神经系统的可能性和致病性提供线索。(C)2020 Elsevier Inc.保留所有权利。
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been discovered as the pathogenic cause of the coronavirus disease 19 (COVID-19). Cellular entry of SARS-CoV-2 are mediated by the spike glycoprotein of virus, and the host specific receptors and proteases. Recently, besides pulmonary complications as the chief symptom, investigations have also revealed that SARS-CoV-2 can trigger neurological manifestations. Herein, to investigate the expression level of receptors and related proteases is important for understanding the neuropathy in COVID-19. We determined the expression levels of receptor ACE2 and CD147, and serine protease TMPRSS2 in human and mouse brain cell lines and mouse different region of brain tissues with qRT-PCR and Western blot. The results showed that the expression pattern of all them was very different to that of lung. ACE2 is lower but CD147 is higher expressed in mostly brain cell lines and mouse brain tissues comparing with lung cell line and tissue, and TMPRSS2 has consistent expression in brain cell lines and mouse lung tissues. It is suggested that SARS-CoV-2 might have a different way of infection to cerebral nervous system. Our finding will offer the clues to predict the possibility of SARS-CoV-2 infection to human brain nervous system and pathogenicity. (C) 2020 Elsevier Inc. All rights reserved.