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DOI：
10.1111/j.1944-8287.2000.tb00151.x
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发表时间：
2000-10
影响因子：
7
通讯作者：
Arifin Musthafa
中科院分区：
文献类型：
--
作者：
Arifin Musthafa
背景：辐射工作人员的辐射暴露电离有可能以双链断裂的形式引起DNA损伤，作为基因组不稳定的开始。γ-H_2AX可作为DNA双链断裂(DSB)的生物标志物。1mGy射线照射后，细胞核内可形成γ-H_2AX。本研究旨在通过观察γ-H_2AX焦点的表达来研究外周血单个核细胞(PBMC)对辐射的适应性反应，以确定辐射作业环境中的辐射效应。方法：对9名放射工作人员和9名非放射工作人员的血样分别进行0、1、1.5、2Gy4个剂量的照射。免疫荧光法检测γ-H_2AX病灶。每例样本50例，计数γ-H_2AX病灶平均数。γ-H_2AX病灶比较均值采用t-独立性检验。结果：两组治疗前γ-H_2AX病灶数目差异无统计学意义(p=0.807)。研究结果表明，2Gy射线照射后，每个细胞形成2~3个病灶的量随照射剂量的增加而增加。结论：1Gy1、1.5Gy2、2Gy3种剂量照射后，非放射工作人员与放射工作人员外周血单核细胞γ-H_2AX病灶指数在正常范围内的平均值和DSB损伤的危险性水平比较接近。
Background: Exposure ionizing of radiation in radiation workers has the potential to cause DNA damage in the form of double strand break as the beginning of genomic instability. DNA damage can be observed with γ-H2AX as the biomarker of DNA double strand breaks (DSBs). The formation of γ-H2AX in the nucleus can occur after radiation exposure of 1 mGy. This study aims to determine the radiation effects in radiation work environments as a study of adaptive responses of peripheral blood mononuclear cell (PBMCs) after radiation by observing γ-H2AX foci expression.. Methods: Blood samples from nine radiation workers and nine non-radiation workers were irradiated with doses 0 Gy, 1 Gy, 1.5 Gy, and 2 Gy. Detection of γ-H2AX foci was done by immunofluorescence assay. The mean of γ-H2AX foci was counted in 50 PBMCs per sample. The comparison mean of γ-H2AX foci was analyzed using tindependent test. Result: Based on the result study, there were no significant differences in the number of γ-H2AX foci without treatment (p = 0.807). The results of study showed that the formation of 2-3 foci per cell after exposure of 2 Gy increases along with the increasing irradiation doses. Conclusion: The mean of index of γ-H2AX foci in PBMCs within normal limits between non-radiation workers and radiation workers and level of risk DSBs damage is relatively similar after exposure at doses 1 Gy, 1.5 Gy, and 2 Gy.