PROGRESSIVE LOSS OF DNA-SEQUENCES FROM TERMINAL CHROMOSOME DEFICIENCES IN DROSOPHILA-MELANOGASTER
PROGRESSIVE LOSS OF DNA-SEQUENCES FROM TERMINAL CHROMOSOME DEFICIENCES IN DROSOPHILA-MELANOGASTER
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DOI：
10.1002/j.1460-2075.1988.tb02916.x
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发表时间：
1988-04-01
期刊：
影响因子：
11.4
通讯作者：
MASON, JM
中科院分区：
文献类型：
--
作者：
BIESSMANN, H;MASON, JM
用低剂量的X射线照射携带纯合突变子（mu-2）的果蝇雌性，可以高频率（0.2-0.3%）诱导X染色体顶端的末端缺陷。这些末端缺陷是不稳定的，因为在3年半的时间内，DNA序列以每代75 bp的速率从其末端丢失，这可能是由于缺乏完整的野生型端粒结构。在黄色基因的5“上游调控区中的这些缺失的断点，产生典型的y2型马赛克角质层色素沉着模式，用于定义身体、翅膀或刚毛色素沉着所需的组织特异性顺式作用调控元件的位置。
Terminal deficiencies at the tip of the X chromosome can be induced at a high frequency (0.2-0.3%) by irradiating Drosophila females carrying a homozygous mutator (mu-2) with low doses of X-rays. These terminal deficiencies are unstable, since over a period of 31/2 years DNA sequences were lost from their distal ends at a rate of 75 bp per generation, presumably due to the absence of a complete wild-type telomeric structure. Breakpoints of these deletions in the 5'' upstream regulatory region of the yellow gene, giving rise to a mosaic cuticle pigmentation pattern typical of the y2 type, were used to define the location of tissue-specific cis-acting regulatory elements that are required for body, wing or bristle pigmentation.