Synchronized oscillations caused by disinhibition in rodent neocortex are generated by recurrent synaptic activity mediated by AMPA receptors
Synchronized oscillations caused by disinhibition in rodent neocortex are generated by recurrent synaptic activity mediated by AMPA receptors
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DOI：
10.1113/jphysiol.2002.019059
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发表时间：
2002-07-15
影响因子：
5.5
通讯作者：
Rigas, P
中科院分区：
文献类型：
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作者：
Castro-Alamancos, MA;Rigas, P
在去抑制过程中，新皮层产生同步活动。在新皮层切片中，GABA(A)和GABA(B)受体拮抗剂的应用将缓慢振荡转化为振幅较大的尖峰波放电，其节奏与10 Hz的新皮层振荡相似。解除抑制引起的10 Hz振荡具有高度的区域特异性，仅在新皮层的额叶颗粒区（如初级运动皮层）产生，而在颗粒状新皮层中不产生。药理学操作表明，10hz振荡严重依赖于α -氨基-3-羟基-5-甲基异恶唑-4-丙酸（AMPA）受体。利用16位硅探针对薄片进行电流源密度（CSD）分析发现，10hz振荡表现为上层的大电流汇和下层的小电流汇。结果表明，阻断颗粒状新皮层中的GABA(B)受体揭示了AMPA受体介导的周期性突触活动，从而导致这些振荡的产生。
During disinhibition the neocortex generates synchronous activities. In neocortical slices application of GABA(A) and GABA(B) receptor antagonists transformed slow oscillations into large amplitude spike-wave discharges that contained a rhythmic similar to10 Hz neocortical oscillation. The 10 Hz oscillations caused by disinhibition were highly region specific and were generated only in frontal agranular regions of neocortex, such as the primary motor cortex, but not in granular neocortex. Pharmacological manipulations showed that the 10 Hz oscillations were critically dependent on alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) receptors. Current source density (CSD) analyses in slices using 16-site silicon probes revealed that the 10 Hz oscillations were expressed with large current sinks in the upper layers and smaller current sinks in the lower layers that precede them. The results indicate that blocking GABA(B) receptors in the agranular neocortex unmasks recurrent synaptic activity mediated by AMPA receptors that results in the generation of these oscillations.