Specific tissue proteins 1 and 6 are involved in root biology during normal development and under symbiotic and pathogenic interactions in Medicago truncatula
Specific tissue proteins 1 and 6 are involved in root biology during normal development and under symbiotic and pathogenic interactions in Medicago truncatula
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DOI：
10.1007/s00425-020-03538-4
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发表时间：
2021-01-02
期刊：
影响因子：
4.3
通讯作者：
Dopico, Berta
中科院分区：
文献类型：
--
作者：
Albornos, Lucia;Casado-del-Castillo, Virginia;Dopico, Berta
主要结论ST1和ST6可能参与初生根和侧根及共生根瘤的发育，但只有ST6参与与半生物营养真菌的相互作用。摘要特定的组织(ST)蛋白参与植物营养状况、发育和对生物制剂的响应等过程。特别是，ST1和ST6主要在植物发育过程中的根中表达。在这里，我们分析了在豆科模式植物紫花苜蓿中，编码这两种蛋白的基因的表达在哪里以及如何调节，以响应植物的发育程序，由有益的固氮菌(Sinorhizobium Meliloti)诱导的结瘤，以及由致病的半生物营养真菌(Fusum Oxporum)引发的防御反应。基因表达结果表明，ST1和ST6参与了初生根和侧根的维管系统发育，但只有ST6与分生组织的活性有关。ST1和ST6在所分析的生物相互作用中明显表现出不同的作用，其中ST1被固氮细菌激活，ST6在接种尖孢镰刀菌后上调。ST1和ST6在结瘤过程中的作用可能与根瘤器官发生有关，而不是相互作用本身的建立，ST6的增加与侵染和定殖过程中水杨酸信号通路的激活有关。这些结果进一步支持了ST6在响应半生物营养真菌中的作用。这项研究有助于理解控制根生物学的复杂网络，并加强了ST蛋白参与了几个过程，如初级和侧根发育、根瘤器官发生以及植物与微生物的相互作用。
Main conclusionST1 and ST6 are possibly involved in primary and lateral root and symbiotic nodule development, but only ST6 participates in the interaction with hemibiotrophic fungi.AbstractSpecific tissue (ST) proteins have been shown to be involved in several processes related to plant nutritional status, development, and responses to biotic agents. In particular, ST1 and ST6 are mainly expressed in roots throughout plant development. Here, we analyze where and how the expression of the genes encoding both proteins are modulated in the legume model plant Medicago truncatula in response to the plant developmental program, nodulation induced by a beneficial nitrogen-fixing bacterium (Sinorhizobium meliloti) and the defense response triggered by a pathogenic hemibiotrophic fungus (Fusarium oxysporum). Gene expression results show that ST1 and ST6 participate in the vasculature development of both primary and lateral roots, although only ST6 is related to meristem activity. ST1 and ST6 clearly display different roles in the biotic interactions analyzed, where ST1 is activated in response to a N-2-fixing bacterium and ST6 is up-regulated after inoculation with F. oxysporum. The role of ST1 and ST6 in the nodulation process may be related to nodule organogenesis rather than to the establishment of the interaction itself, and an increase in ST6 correlates with the activation of the salicylic acid signaling pathway during the infection and colonization processes. These results further support the role of ST6 in response to hemibiotrophic fungi. This research contributes to the understanding of the complex network that controls root biology and strengthens the idea that ST proteins are involved in several processes such as primary and lateral root development, nodule organogenesis, and the plant-microbe interaction.