Multiwalled carbon nanotube-coating of 3D collagen scaffolds for bone tissue engineering
Multiwalled carbon nanotube-coating of 3D collagen scaffolds for bone tissue engineering
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DOI：
10.1016/j.carbon.2011.04.002
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发表时间：
2011-08-01
期刊：
影响因子：
10.9
通讯作者：
Yokoyama, Atsuro
中科院分区：
文献类型：
--
作者：
Hirata, Eri;Uo, Motohiro;Yokoyama, Atsuro
采用多壁碳纳米管（MWCNTs）对骨组织工程用三维胶原支架进行表面包覆处理。大鼠原代成骨细胞（ROBs）培养在MWCNT涂层胶原海绵（MWCNT涂层海绵）在三维动态流动细胞培养系统和分化标志物进行了测量。碱性磷酸酶活性在1天，钙和骨桥蛋白含量的多壁碳纳米管涂层海绵在7天显着高于未涂层海绵。多壁碳纳米管涂层海绵上的ROB比未涂层海绵上的ROB更早分化。此外，评价了对MWCNT涂覆的海绵的组织反应。在皮下组织中植入后第7天和第28天，在MWCNT周围观察到轻微炎症。在植入股骨后28天和56天，观察到多壁碳纳米管涂层海绵周围的骨形成明显多于未涂层海绵周围的骨形成和直接附着在多壁碳纳米管上的新骨。此外，在植入具有培养1天的成骨细胞的MWCNT涂覆的海绵后28天，根据其蜂窝结构，骨组织成功地在孔中形成。因此，多壁碳纳米管涂层似乎是有效的骨组织工程。(C)2011爱思唯尔有限公司版权所有。
Surface coating treatment with multiwalled carbon nanotubes (MWCNTs) was applied to 3D collagen scaffold for bone tissue engineering. Rat primary osteoblasts (ROBs) were cultured on an MWCNT-coated collagen sponge (MWCNT-coated sponge) in a 3D dynamic flow cell culture system and differentiation markers were measured. Alkaline phosphatase activity at 1 day, and calcium and osteopontin contents of the MWCNT-coated sponges at 7 days were significantly higher than those of uncoated sponges. ROBs on the MWCNT-coated sponge differentiated earlier than on the uncoated sponge. In addition, the tissue response to the MWCNT-coated sponge was evaluated. Slight inflammation was observed around MWCNTs at 7 and 28 days after implantation in subcutaneous tissue. Significantly more bone formation was observed around the MWCNT-coated sponges than around the uncoated sponges and new bone attached to MWCNTs directly at 28 and 56 days after implantation in the femur. Moreover, at 28 days after implantation of the MWCNT-coated sponge with osteoblasts cultured for 1 day, bone tissues were successfully formed in the pores according to its honeycomb structure. Therefore, MWCNT coating appears to be effective for bone tissue engineering. (C) 2011 Elsevier Ltd. All rights reserved.