PACTAMYCIN PRODUCTION BY STREPTOMYCES PACTUM
PACTAMYCIN PRODUCTION BY STREPTOMYCES PACTUM
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DOI：
10.1128/aem.10.4.302-304.1962
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发表时间：
1962-01-01
期刊：
影响因子：
--
通讯作者：
BHUYAN, BK
中科院分区：
文献类型：
--
作者：
BHUYAN, BK
研究了一株新型抗肿瘤抗生素--帕克霉素产生菌Streptomyces pactum var. pactum进行了调查。最适生长pH范围为6.5 ~ 7.0。该菌生长和产生抗生素的最适温度为。在含有Cerelose、黑糖蜜、Pabst酵母、Kay大豆、CaCO 3和KCl的培养基中研究。由于在32 ℃下获得最大生长和最大生产效率，所有后续发酵均在此温度下进行。根据发酵条件的不同，与菌丝体结合的抗生素的量不同，并且可以用丙酮提取。在含有Cerelose、大豆蛋白胨、碳酸钙和氯化钾的培养基中，可以获得良好的产率（216 [mu]g/ml）。发酵过程中的生化变化分析表明，在后期自溶阶段产生的帕塔霉素。
The optimal fermentation conditions for the production of pactamycin (a new antitumor antibiotic) by Streptomyces pactum var. pactum were investigated. The optimal pH range for growth was 6.5 to 7.0. The optimal temperature for the growth of the culture and the production of the antibiotic was . investigated in a medium containing Cerelose, blackstrap molasses, Pabst yeast, Kay Soy, CaCO3 and KC1. Since maximal growth and maximal production efficiency was obtained at 32 C, all subsequent fermentations were conducted at this temperature. Pactamycin was bound to the mycelium in different amounts depending on the fermentation conditions and could be extracted with acetone. Good yields (216 [mu]g/ml) of pactamycin could be obtained in a medium containing Cerelose, soy-peptone, calcium carbonate and potassium chloride. Analysis of the biochemical changes during fermentation indicated that pactamycin was produced during the later autolytic phase.