JA-Induced Endocytosis of AtRGS1 Is Involved in G-Protein Mediated JA Responses
JA-Induced Endocytosis of AtRGS1 Is Involved in G-Protein Mediated JA Responses
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JA 诱导的 AtRGS1 内吞作用参与 G 蛋白介导的 JA 反应
DOI：
10.3390/ijms20153779
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发表时间：
2019-08-01
影响因子：
5.6
通讯作者：
Shan, Xiaoyi
中科院分区：
文献类型：
--
作者：
Li, Li;Su, Bodan;Shan, Xiaoyi
拟南芥异源三聚体G蛋白通过与7 TM AtRGS 1蛋白偶联来调节植物的生长和防御过程。虽然G蛋白突变体显示响应于多种植物激素的改变，但G蛋白参与调节激素反应的潜在机制仍然难以捉摸。在这里，我们表明，G α和G β亚基的遗传破坏导致对JA治疗的敏感性降低。此外，使用共聚焦显微镜，VA-TIRFM，和FRET-FLIM，我们提供的证据表明，刺激JA诱导磷酸化和C-末端依赖的内吞AtRGS 1，然后促进AtRGS 1从AtGPA 1的解离。此外，SPT分析表明，JA处理影响AtRGS 1和AtRGS 1-Delta Ct的扩散动力学。总之，这些发现表明，JA信号激活异源三聚体G蛋白通过AtRGS 1的内吞作用和AtRGS 1从AtGPA 1的解离，从而提供了有价值的洞察G蛋白系统如何感知和转导植物激素信号的机制。
Arabidopsis heterotrimeric G proteins regulate diverse plant growth and defense processes by coupling to 7TM AtRGS1 proteins. Although G protein mutants display alterations in response to multiple plant hormones, the underlying mechanism by which G proteins participate in the regulation of hormone responses remains elusive. Here, we show that genetic disruption of G alpha and G beta subunits results in reduced sensitivity to JA treatment. Furthermore, using confocal microscopy, VA-TIRFM, and FRET-FLIM, we provide evidence that stimulation by JA induces phosphorylation- and C-terminus-dependent endocytosis of AtRGS1, which then promotes dissociation of AtRGS1 from AtGPA1. In addition, SPT analysis reveals that JA treatment affects the diffusion dynamics of AtRGS1 and AtRGS1-Delta Ct. Taken together, these findings suggest that the JA signal activates heterotrimeric G proteins through the endocytosis of AtRGS1 and dissociation of AtRGS1 from AtGPA1, thus providing valuable insight into the mechanisms of how the G protein system perceives and transduces phytohormone signals.