Sulfurospirillum diekertiae sp. nov., a tetrachloroethene- respiring bacterium isolated from contaminated soil
Sulfurospirillum diekertiae sp. nov., a tetrachloroethene- respiring bacterium isolated from contaminated soil
复制标题
硫螺菌 dikertiae sp.
DOI：
10.1099/ijsem.0.005693
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发表时间：
2023-01-01
影响因子：
2.8
通讯作者：
Yan，Jun
中科院分区：
文献类型：
--
作者：
Jin，Huijuan;Huo，Leitao;Yan，Jun
采用多相法对两株四氯乙烯（PCE）呼吸性厌氧细菌acsdcet1和ACSTCE进行了鉴定。细胞革兰氏染色阴性，可运动，无孢子形成，具有弧菌状至螺旋状形态。在30°C和0.1 - 0.4%盐度条件下生长最佳。生长的pH范围为pH 5.5 ~ 7.5, pH 7.2为最佳。氢、甲酸盐、丙酮酸盐和乳酸盐作为电子供体支持菌株acsdce呼吸还原脱氯生成1,2-二氯乙烯（cDCE）和菌株ACSTCE呼吸还原脱氯生成三氯乙烯（TCE）。这两种菌株都能在丙酮酸的微氧条件下生长。硝酸盐、单质硫和硫代硫酸盐是可选的电子受体。两株菌株均未见自养生长，以乙酸为碳源。主要细胞脂肪酸为C16: 1ω7c、C16: 0、C14: 0和C18: 1ω7c。两个基因组都有一个环状质粒。菌株acsdcet6和acstce2先前被指定为候选种“酸化脱卤硫螺旋菌”。本文基于关键基因组特征和全基因组序列的两两比较，包括平均核苷酸同源性、数字DNA-DNA杂交和平均氨基酸同源性，对菌株acsdcet1和ACSTCE、Ca. Sulfurospirillum diekertiae菌株SL2-1和SL2-2以及未分类的sulfurospirillumsp进行了比较。菌株SPD-1被归类为一个不同的物种，与先前描述的硫螺旋体物种分开。与没有大量TCE积累的多源硫螺旋菌和卤代硫螺旋菌将PCE脱氯到cdce相比，这5个菌株最终产生TCE或cdce。此外，在acsdcet和acstce菌株中检测到一些细胞脂肪酸（如c16:03 oh， c17:0 iso 3OH, c17:02 oh），而在其他硫螺菌株中未检测到。在系统发育、生理和表型特征的基础上，酸化脱卤硫螺旋藻‘和’ ca。diekertiae’被提议合并为sulfurrospirillum diekertiaesp属内的一个新种，因此命名为sulfurrospirillum diekertiaesp。11月被提议。型应变为ACSDCET（=JCM 33349T= KCTC 15819T=CGMCC 1.5292T）。
Two anaerobic, tetrachloroethene- (PCE-) respiring bacterial isolates, designated strain ACSDCETand strain ACSTCE, were characterized using a polyphasic approach. Cells were Gram-stain-negative, motile, non-spore-forming and shared a vibrioid- to spirillum-shaped morphology. Optimum growth occurred at 30 °C and 0.1–0.4 % salinity. The pH range for growth was pH 5.5–7.5, with an optimum at pH 7.2. Hydrogen, formate, pyruvate and lactate as electron donors supported respiratory reductive dechlorination of PCE tocis-1,2-dichloroethene (cDCE) in strain ACSDCETand of PCE to trichloroethene (TCE) in strain ACSTCE. Both strains were able to grow with pyruvate under microaerobic conditions. Nitrate, elemental sulphur, and thiosulphate were alternative electron acceptors. Autotrophic growth was not observed and acetate served as carbon source for both strains. The major cellular fatty acids were C16 : 1ω7c, C16 : 0, C14 : 0and C18 : 1ω7c. Both genomes feature a circular plasmid. Strains ACSDCETand ACSTCEwere previously assigned to the candidate species 'Sulfurospirillum acididehalogenans'. Here, based on key genomic features and pairwise comparisons of whole-genome sequences, including average nucleotide identity, digital DNA–DNA hybridization and average amino acid identity, strains ACSDCETand ACSTCE, 'Ca. Sulfurospirillum diekertiae' strains SL2-1 and SL2-2, and the unclassifiedSulfurospirillumsp. strain SPD-1 are grouped into one distinct species separate from previously describedSulfurospirillumspecies. Compared toSulfurospirillum multivoransandSulfurospirillum halorespirans, which dechlorinate PCE tocDCE without substantial TCE accumulation, these five strains produce TCE orcDCE as the end product. In addition, some cellular fatty acids (e.g., C16 : 03OH, C17 : 0iso 3OH, C17 : 02OH) were detected in strains ACSDCETand ACSTCEbut not in otherSulfurospirillumspecies. On the basis of phylogenetic, physiological and phenotypic characteristics,'Ca. Sulfurospirillum acididehalogenans' and'Ca. Sulfurospirillum diekertiae' are proposed to be merged into one novel species within the genusSulfurospirillum, for which the nameSulfurospirillum diekertiaesp. nov. is proposed. The type strain is ACSDCET(=JCM 33349T= KCTC 15819T=CGMCC 1.5292T).