Embryonic and larval development of Pinctada margaritifera (Linnaeus, 1758).
Embryonic and larval development of Pinctada margaritifera (Linnaeus, 1758).
复制标题
DOI：
10.1071/mr02015
复制
发表时间：
2003-01-01
影响因子：
1
通讯作者：
Southgate, Paul C.
中科院分区：
文献类型：
--
作者：
Doroudi, Mehdi S.;Southgate, Paul C.
我们描述了黑唇珍珠贝、Pinctada margaritifera (Linnaeus, 1758) 幼虫在 28+-1°C 实验室中从受精到胚胎发育和幼虫生长的发育阶段。将幼虫麻醉、固定、临界点干燥并使用扫描电子显微镜进行检查。我们检查了胚胎发育（受精、极体、卵裂球、原肠胚）和幼虫壳的属性（大小、原壳 I/II、生长线、原齿、壳破裂）和幼虫帆。受精后24分钟第一极体形成，受精卵平均直径为59.9+-1.4μm。最早活跃游动的支轮出现在受精后8-12小时。受精后约 24 小时达到 D 阶段，壳长测量为 79.7+-2.3 mm。 10天龄的幼虫在铰链轴上方具有彼此相对的伞骨，22天龄的幼虫平均壳长为230.8+-4.9mm，在进入pediveliger阶段之前出现色素斑点。
We describe the developmental stages of the black-lip pearl oyster, Pinctada margaritifera (Linnaeus, 1758), larvae from fertilisation through embryonic development and larval growth in the laboratory at 28+-1degreeC. Larvae were anesthetised, fixed, critical-point dried and examined using a scanning electron microscope. We examined embryonic development (fertilisation, polar body, blastomeres, gastrula) and attributes of the larval shell (size, prodissoconch I/II, growth lines, provinculum, shell fracture) and larval velum. The first polar body formed 24 min after fertilisation and fertilised eggs had a mean diameter of 59.9+-1.4 mum. The earliest actively swimming trochophore appeared 8-12 h after fertilisation. The D stage was reached approximately 24 h after fertilisation and measured 79.7+-2.3 mum in shell length. Ten-day-old larvae had umbones that arose opposite each other above the hinge axis and 22-day-old larvae, with a mean shell length of 230.8+-4.9 mum, developed a pigment spot just before entering the pediveliger stage.