Axon Outgrowth of Rat Embryonic Hippocampal Neurons in the Presence of an Electric Field.
Axon Outgrowth of Rat Embryonic Hippocampal Neurons in the Presence of an Electric Field.
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DOI：
10.1021/acschemneuro.6b00191
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发表时间：
2016-08
影响因子：
5
通讯作者：
Kwang-Min Kim;Sung Yeol Kim;G. Palmore
中科院分区：
文献类型：
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作者：
Kwang-Min Kim;Sung Yeol Kim;G. Palmore
长期以来，电场（EF）的应用一直被用来诱导神经损伤后的轴突生长。然而，哺乳动物神经元中枢神经系统 (CNS) 对 EF 的反应（例如轴突长度、轴突引导）仍不清楚，而两栖动物或鸟类神经元模型的反应已得到很好的表征。因此，为了确定哺乳动物 CNS 神经元轴突生长的最佳 EF，我们对大鼠海马神经元应用了广泛的 EF。我们的结果表明，高强度（100 mV/mm 或更高）的 EF 或低强度（10-30 mV/mm）长暴露时间（10 小时或更长）的 EF 会导致神经突塌陷和细胞死亡。我们还通过在体外 (DIV) 1 至 11 天对细胞施加 30 mV/mm 的电压，研究了神经元对 EF 的反应是否会根据神经元培养物的生长阶段而改变。当电刺激 4-5 DIV 之间时，神经元显示出轴突生长模式的更新，此时神经元同时形成轴突和树突。这项研究的结果表明，当使用 EF 作为哺乳动物 CNS 神经元轴突再生的潜在方法时，神经元的发育阶段是一个需要考虑的重要因素。
Application of an electric field (EF) has long been used to induce axon outgrowth following nerve injuries. The response of mammalian neurons (e.g., axon length, axon guidance) from the central nervous system (CNS) to an EF, however, remains unclear, whereas those from amphibian or avian neuron models have been well characterized. Thus, to determine an optimal EF for axon outgrowth of mammalian CNS neurons, we applied a wide range of EF to rat hippocampal neurons. Our results showed that EF with either a high magnitude (100 mV/mm or higher) or long exposure time (10 h or longer) with low magnitude (10-30 mV/mm) caused a neurite collapse and cell death. We also investigated whether neuronal response to an EF is altered depending on the growth stage of neuron cultures by applying 30 mV/mm to cells from 1 to 11 days in vitro (DIV). Neurons showed the turnover of axon outgrowth pattern when electrically stimulated between 4-5 DIV at which point neurons have both axonal and dendritic formation. The findings of this study suggest that the developmental stage of neurons is an important factor to consider when using EF as a potential method for axon regeneration in mammalian CNS neurons.