The impact of various reactive oxygen species on the formation of neutrophil extracellular traps.
The impact of various reactive oxygen species on the formation of neutrophil extracellular traps.
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DOI：
10.1155/2012/849136
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发表时间：
2012
影响因子：
4.6
通讯作者：
Behnen M
中科院分区：
文献类型：
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作者：
Kirchner T;Möller S;Klinger M;Solbach W;Laskay T;Behnen M
中性粒细胞胞外陷阱（NETs）的形成依赖于活性氧（ROS）的产生。以往的研究表明，NADPH氧化酶和髓过氧化物酶（MPO）都需要NET的释放。然而，迄今为止，各种活性氧的贡献以及线粒体来源的活性氧的作用尚未得到解决。在本研究中，我们的目的是调查在一个系统和全面的方式的各种ROS和ROS生成途径的PMA诱导NET释放的贡献。通过使用特异性抑制剂，评估NADPH氧化酶和谷胱甘肽衍生的ROS的作用以及超氧化物歧化酶（SOD）和MPO对NET释放的贡献。我们可以证明，NADPH氧化酶的功能是至关重要的NET的形成。此外，我们可以清楚地表明MPO衍生的ROS参与NET的释放。然而，我们的研究结果并没有提供证据的作用，SOD或ESTA衍生的ROS NET的形成。
The formation of neutrophil extracellular traps (NETs) depends on the generation of reactive oxygen species (ROS). Previous studies revealed that both NADPH oxidase and myeloperoxidase (MPO) are required for NET release. However, the contribution of various ROS as well as the role of mitochondria-derived ROS has not been addressed so far. In the present study we aimed to investigate in a systematic and comprehensive manner the contribution of various ROS and ROS-generating pathways to the PMA-induced NET release. By using specific inhibitors, the role of both NADPH oxidase- and mitochondria-derived ROS as well as the contribution of superoxide dismutase (SOD) and MPO on the NET release was assessed. We could demonstrate that NADPH oxidase function is crucial for the formation of NETs. In addition, we could clearly show the involvement of MPO-derived ROS in NET release. Our results, however, did not provide evidence for the role of SOD- or mitochondria-derived ROS in NET formation.
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影响因子：
5.8
作者：
KETTLE, AJ;WINTERBOURN, CC
通讯作者：
WINTERBOURN, CC
影响因子：
4.8
作者：
Li, NY;Ragheb, K;Robinson, JP
通讯作者：
Robinson, JP
影响因子：
2.2
作者：
Hasegawa, H;Suzuki, K;Sugawara, K
通讯作者：
Sugawara, K
影响因子：
5.5
作者：
Narayanan, PK;Carter, WO;Robinson, JP
通讯作者：
Robinson, JP
影响因子：
82.9
作者：
通讯作者：
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