Targeting human immunodeficiency virus (HIV) type 2 integrase protein into HIV type 1.
Targeting human immunodeficiency virus (HIV) type 2 integrase protein into HIV type 1.
复制标题
将人类免疫缺陷病毒 (HIV) 2 型整合酶蛋白靶向转化为 HIV 1 型。
DOI：
10.1128/jvi.73.10.8831-8836.1999
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发表时间：
1999
影响因子：
5.4
通讯作者：
Kappes,JC
中科院分区：
文献类型：
--
作者：
Liu,H;Wu,X;Xiao,H;Kappes,JC
Integrase (IN) is the only retroviral enzyme necessary for the integration of retroviral cDNA into the host cell’s chromosomes. The structure and function of IN is highly conserved. The human immunodeficiency virus type 2 (HIV-2) IN has been shown to efficiently support 3′ processing and strand transfer of HIV-1 DNA substrate in vitro. To determine whether HIV-2 IN protein (IN2) could substitute for HIV-1 IN function in vivo, we used HIV-1 Vpr to deliver the IN2into IN mutant HIV-1 virions by expression intransas a Vpr-IN fusion protein.Trans-complementation with IN2markedly increased the infectivity of IN-minus HIV-1. Compared with the homologoustrans-IN protein, infectivity was increased to a level of 16%. Since IN has been found to play a role in reverse transcription (Wu et al., J. Virol. 73:2126–2135, 1999), cells infected with IN2-complemented HIV-1 were analyzed for DNA products of reverse transcription. DNA levels of approximately 18% of that of wild type were detected. The homologoustrans-IN protein restored the synthesis of viral cDNA to approximately 86% of that of wild-type virus. By complementing integration-defective HIV-1 IN mutant viruses, which were not impaired in cDNA synthesis, thetrans-IN2protein was shown to support integration up to a level of 55% compared with that of the homologoustrans-IN protein. The delivery of heterologous IN protein into HIV-1 particles intransoffers a novel approach to understand IN protein function in vivo.
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用 IL-2 和抗 T3 诱导后，正常 T 细胞同时产生肿瘤坏死因子-α 和淋巴毒素。
DOI：
--
发表时间：
1988
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Steffen,M;Ottmann,OG;Moore,MA
通讯作者：
Moore,MA
OKT3单克隆抗体激活T细胞的要求：T3分子和白细胞介素1的调节作用。
DOI：
--
发表时间：
1985
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Schwab,R;Crow,MK;Russo,C;Weksler,ME
通讯作者：
Weksler,ME
重组肿瘤坏死因子和重组白细胞介素2联合肿瘤免疫治疗小鼠
影响因子：
6.4
作者：
T. Nishimura;So Ohta;N. Sato;Y. Togashi;M. Goto;Y. Hashimoto
通讯作者：
Y. Hashimoto
影响因子：
4.1
作者：
GEORGE, RE;LOUDON, WG;GRIMM, EA
通讯作者：
GRIMM, EA
影响因子：
4.4
作者：
A. Ochoa;G. Gromo;B. Alter;P. Sondel;F. Bach
通讯作者：
A. Ochoa;G. Gromo;B. Alter;P. Sondel;F. Bach