Genetic map of randomly amplified DNA polymorphisms closely linked to the mating type locus of Tetrahymena thermophila.
Genetic map of randomly amplified DNA polymorphisms closely linked to the mating type locus of Tetrahymena thermophila.
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DOI：
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发表时间：
1995-12
期刊：
影响因子：
3.3
通讯作者：
T. Lynch;J. Brickner;K. Nakano;E. Orias
中科院分区：
文献类型：
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作者：
T. Lynch;J. Brickner;K. Nakano;E. Orias
我们使用基于PCR的随机扩增多态性DNA（RAPD）方法，有效地鉴定和定位的DNA多态性的纤毛虫四膜虫thermophila。多态性分离孟德尔遗传标记。一个有针对性的屏幕，使用合并的减数分裂分离的DNA，产生的多态性最紧密连锁的垫基因座。已经鉴定了与2号微核染色体左臂的mat-Pmr片段连锁的总共10个多态性。这构成了T.嗜热菌我们还提供了在垫区域，20 kb/cM的减数分裂重组的频率的第一个粗略估计。这一频率远远高于在大多数其他真核生物中观察到的频率。四膜虫遗传学的特殊功能增强了RAPD方法的能力：能够在一个单一的步骤中获得减数分裂分离，是全基因组纯合子和缺体株允许快速删除多态性的微核染色体或染色体片段的映射。RAPD方法似乎提供了一个实用的和相对便宜的方法来构建一个高分辨率的四膜虫基因组图谱。
We have used the PCR-based randomly amplified polymorphic DNA (RAPD) method to efficiently identify and map DNA polymorphisms in the ciliated protozoan Tetrahymena thermophila. The polymorphisms segregate as Mendelian genetic markers. A targeted screen, using DNA from pooled meiotic segregants, yielded the polymorphisms most closely linked to the mat locus. A total of 10 polymorphisms linked to the mat-Pmr segment of the left arm of micronuclear chromosome 2 have been identified. This constitutes the largest linkage group described in T. thermophila. We also provide here the first crude estimate of the frequency of meiotic recombination in the mat region, 20 kb/cM. This frequency is much higher than that observed in most other eukaryotes. Special features of Tetrahymena genetics enhanced the power of the RAPD method: the ability to obtain in a single step meiotic segregants that are whole-genome homozygotes and the availability of nullisomic strains permitting quick deletion mapping of polymorphisms to micronuclear chromosomes or chromosome segments. The RAPD method appears to provide a practical and relatively inexpensive approach to the construction of a high-resolution map of the Tetrahymena genome.