PHYLOGENETIC INFORMATION-CONTENT OF 5 NUCLEAR GENE-SEQUENCES IN ANIMALS - INITIAL ASSESSMENT OF CHARACTER SETS FROM CONCORDANCE AND DIVERGENCE STUDIES
PHYLOGENETIC INFORMATION-CONTENT OF 5 NUCLEAR GENE-SEQUENCES IN ANIMALS - INITIAL ASSESSMENT OF CHARACTER SETS FROM CONCORDANCE AND DIVERGENCE STUDIES
复制标题
DOI：
10.2307/2413549
复制
发表时间：
1994-12-01
影响因子：
6.5
通讯作者：
MITTER, C
中科院分区：
文献类型：
--
作者：
FRIEDLANDER, TP;REGIER, JC;MITTER, C
编码氨基酸序列的五个核基因的系统发育信息内容是根据它们对已建立的“测试”系统发育的特征支持以及它们使用简约方法恢复这些系统发育的能力来评估的。这五个基因编码多巴脱羧酶、磷酸烯醇丙酮酸羧激酶、RNA 聚合酶 II（最大亚基）、延伸因子 2 和延伸因子 1 α。这些分析采用了所有可用动物序列中这些基因的明确可比对部分，代表了从大约 10 到 > 5.5 亿年前的一系列分歧时间。使用按密码子位置分隔的核苷酸和氨基酸进行单独的分析，包括测试系统发育的字符映射，以及不同时在最简约树上的字符映射。计算所有比较的成对序列分歧，并且与字符信息内容相关。氨基酸和大多数核苷酸字符集恢复了测试系统发育，尽管具有不同程度的支持、同质性和分辨率，证实这些基因包含大量的系统发育信息。提供了对每个基因中的每个字符集将在系统发育上提供最多信息的时间跨度的估计。这些基因应该被证明对系统学有广泛的用途。
The phylogenetic information content of five nuclear genes encoding amino acid sequences was assessed on the basis of their character support of well-established ''test'' phylogenies and their ability to recover these phylogenies using parsimony methods. The five genes encode dopa decarboxylase, phosphoenolpyruvate carboxykinase, RNA polymerase II (largest subunit), elongation factor-2, and elongation factor-1 alpha. The analyses employ the unambiguously alignable portions of all available animal sequences for these genes, representing a range of divergence times from about 10 to > 550 million years ago. Separate analyses, including character mappings on the test phylogenies and, when different, on the most-parsimonious trees, were performed with nucleotides separated by codon position and with amino acids. Pairwise sequence divergences were calculated for all comparisons and are related to character information content. Amino acid and most nucleotide character sets recovered the test phylogenies, although with varying degrees of support, homoplasy, and resolution, confirming that these genes contain substantial phylogenetic information. An estimate is provided of the time span over which each character set in each gene will prove phylogenetically most informative. These genes should prove widely useful for systematics.