High Levels of Genetic Diversity in Salix viminalis of the Czech Republic as Revealed by Microsatellite Markers

High Levels of Genetic Diversity in Salix viminalis of the Czech Republic as Revealed by Microsatellite Markers
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微卫星标记揭示捷克共和国柳树的高水平遗传多样性

DOI:
10.1007/s12155-012-9212-4
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发表时间:
2012
期刊:
影响因子:
3.6
通讯作者:
Trybush S
Trybush S
中科院分区:
工程技术3区
文献类型:
--
作者:
Trybush S

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Willows (Salixspp.) grown as short rotation coppice are recognised as an important bioenergy crop, and breeding programmes are underway in several countries, including the Czech Republic. The basket willowSalix viminalisis one of the few willow species that is widespread in the Czech Republic and thus a potential source of diversity, but the most extensive germplasm collection available shows evidence of redundancy. To investigate levels of variation in natural populations of this species for use in crop improvement programmes, a set of 38 microsatellite markers was used to assess genetic diversity and population structure among 84S. viminalisindividuals collected from seven Czech rivers (the Odra, Bečva, Morava, Dyje, Jihlava, Sázava and Vltava), covering a wide geographic distribution. The markers detected 6.95 alleles per locus on average with 92 % of the sampled individuals having a unique multilocus genotype giving a high clonal richness measure among all samples (R= 0.952). Three sets of putative clones (with identical genotypes as determined by the markers used here) were also identified. Significant levels of genetic diversity were revealed within all sampling sites. With the exception of sites on the Odra and Morava, pairwiseFST(0.02–0.1) values indicated moderate differentiation between sites. Principal coordinates analysis revealed some separation of the Dyje individuals from all others. This was in agreement with the population structure results derived from Bayesian analyses using STRUCTURE software. These results provide the first evidence that potentially useful levels of genotypic variation are present within naturalS. viminalispopulations in the Czech Republic.
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发表时间: 2000-05
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