The effect of matrix composition of 3D constructs on embryonic stem cell differentiation
The effect of matrix composition of 3D constructs on embryonic stem cell differentiation
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DOI：
10.1016/j.biomaterials.2005.04.003
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发表时间：
2005-11-01
期刊：
影响因子：
14
通讯作者：
Netti, PA
中科院分区：
文献类型：
--
作者：
Battista, S;Guarnieri, D;Netti, PA
胚胎干细胞作为无限的细胞来源在组织工程中的应用点燃了体外再生任何组织的希望。然而，该材料在控制和引导其发育和转化为复杂和可行的三维（3D）组织中的作用仍然知之甚少。在这项工作中，我们调查的材料组成和结构对促进ES细胞的生长和分化的作用，通过培养小鼠ES细胞衍生的胚状体（EBs）在各种半互穿聚合物网络（SIPNs），由胶原蛋白，纤连蛋白（FN）和层粘连蛋白（LM）。我们发现，支持矩阵的组成和强度在EB的发展中起着重要的作用。高胶原蛋白浓度通过抑制细胞凋亡来抑制EB空化并因此抑制随后的EB分化。FN在3D胶原构建物中的存在强烈刺激内皮细胞分化和血管形成。相反，LM增加ES细胞分化为搏动心肌细胞的能力。我们的数据表明，基质成分在EB的发展中具有重要作用，并且通过调节支架的物理和生化特性，可以影响干细胞向优先模式分化。(c)2005爱思唯尔有限公司保留所有权利。
The use of embryonic stem (ES) cells as unlimited cell source in tissue engineering has ignited the hope of regenerating any kind of tissue in vitro. However, the role of the material in control and guidance of their development and commitment into complex and viable three-dimensional (3D) tissues is still poorly understood. In this work, we investigate the role of material composition and structure on promoting ES cells growth and differentiation, by culturing mouse ES cell-derived embryoid bodies (EBs) in various semi-interpenetrating polymer networks (SIPNs), made of collagen, fibronectin (FN) and laminin (LM). We show that both composition and strength of the supportive matrix play an important role in EBs development. High collagen concentrations inhibit EBs cavitation and hence the following EBs differentiation, by inhibiting apoptosis. The presence of FN in 3D collagen constructs strongly stimulates endothelial cell differentiation and vascularization. Conversely, LM increases the ability of ES cells to differentiate into beating cardiomyocytes. Our data suggest that matrix composition has an important role in EBs development and that it is possible to influence stem cell differentiation toward preferential pattern, by modulating the physical and biochemical properties of the scaffold. (c) 2005 Elsevier Ltd. All rights reserved.