Lipid interactions in membranes of extremely halophilic bacteria. I. Electron spin resonance and dilatometric studies of bilayer structure.
Lipid interactions in membranes of extremely halophilic bacteria. I. Electron spin resonance and dilatometric studies of bilayer structure.
复制标题
极度嗜盐细菌膜中的脂质相互作用。
DOI：
10.1021/bi00721a005
复制
发表时间：
1974
期刊：
影响因子：
2.9
通讯作者：
M. Kates
中科院分区：
文献类型：
--
作者：
W. Plachy;J. Lanyi;M. Kates
用氮氧化物标记硬脂酸和硫酸钒自旋标记的体积膨胀法和电子自旋共振(ESR)自旋标记法研究了皮肤嗜盐杆菌极性脂在缓冲液和盐溶液中的分散情况。极性脂肪组分本质上是磷脂酰甘油磷酸二植醚类似物和糖脂硫酸盐的二元混合物，摩尔比约为2：1。硬脂酸自旋标记物也用于研究每个纯的主要组分。这些脂类的不同寻常的化学特征包括均匀的、高度支化的植酸基碳氢化合物链、大的阴离子头基以及与植酸基链的乙醚连接。在添加盐(4IVT氯化钠和/或0.1M氯化镁)存在的情况下，脂类的膨胀热系数随着温度的升高而降低
Dispersions of Halobacterium cutirubrum polar lipids in buffer and in salt solutions have been investi-gated over a wide temperature range (but above the phase transition) by volumetric dilatometry and electron spin res-onance(esr) spin-labeling with nitroxide-labeled stearic acids and with vanadyl sulfate. The polar lipid fraction is essentially a binary mixture of the diphytanyl ether analogs of phosphatidyl glycerophosphate and of a glycolipid sulfate, in an approximate mole ratio of 2: 1. Stearic acid spinlabels were used to study each pure major component as well. The unusual chemical features of these lipids include uniform, highly branched phytanyl hydrocarbon chains, large anionic head groups, and ether linkages to the phytanyl chains. The thermal coefficient of expansion of the lipids in the presence of added salts (4 ivt NaCl and/or 0.1 M MgCL) was found to decrease with increasing temperature