Fabrication of Multilayer Microfluidic Arrays for Passive, Efficient DNA Trapping and Profiling.
Fabrication of Multilayer Microfluidic Arrays for Passive, Efficient DNA Trapping and Profiling.
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用于被动、高效 DNA 捕获和分析的多层微流控阵列的制造。
DOI：
10.1007/978-1-0716-3271-0_22
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Wang,Tza-HueiJeff
中科院分区：
文献类型：
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作者：
O'Keefe,ChristineM;Wang,Tza-HueiJeff
在血浆中可以发现含有癌症特异性生物标志物的痕量游离DNA。这些生物标志物的检测具有巨大的应用潜力，如非侵入性癌症诊断和治疗监测。然而，这样的DNA分子是极其罕见的，并且典型的患者血液样本可能仅含有几个拷贝。在这里，我们描述了一种微流体装置的制造和操作，以有效地将单个DNA分子捕获到腔室中，通过被动的几何操作策略检测肿瘤特异性生物标志物。
Trace amounts of cell-free DNA containing cancer-specific biomarkers can be found in blood plasma. Detection of these biomarkers holds tremendous potential for applications such as noninvasive cancer diagnostics and therapeutic monitoring. However, such DNA molecules are extremely rare, and a typical patient blood sample may only contain a few copies. Here we describe the fabrication and operation of a microfluidic device to efficiently trap single DNA molecules into chambers for detection of tumor-specific biomarkers through a passive, geometric manipulation strategy.