Testin is a tumor suppressor in non-small cell lung cancer
Testin is a tumor suppressor in non-small cell lung cancer
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DOI：
10.3892/or.2016.5316
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发表时间：
2017-02-01
期刊：
影响因子：
4.2
通讯作者：
Huang, Li-Nian
中科院分区：
文献类型：
--
作者：
Wang, Ming;Wang, Qian;Huang, Li-Nian
Testin基因先前在7q31.2的脆性染色体区域FRA7G中鉴定。它与几种类型的癌症有关，包括前列腺癌、卵巢癌、乳腺癌和胃癌。在本研究中，我们研究了候选肿瘤抑制基因Testin在非小细胞肺癌（NSCLC）中的功能。在NSCLC细胞系中，我们观察到Testin的表达低于正常人支气管上皮细胞中的表达。MTT法、流式细胞术、克隆形成实验和侵袭实验表明，Testin基因过表达抑制癌细胞的增殖、侵袭和克隆形成。在肿瘤异种移植模型中，Testin显著抑制肺癌细胞在无胸腺裸鼠中的异种移植物形成和生长。综上所述，这些数据表明Testin在NSCLC的发生和进展中起重要作用。睾丸素可能成为NSCLC预防和治疗的新靶点。
The Testin gene was previously identified in the fragile chromosomal region FRA7G at 7q31.2. It has been implicated in several types of cancers including prostate, ovarian, breast and gastric cancer. In the present study, we investigated the function of the candidate tumor-suppressor Testin gene in non-small cell lung cancer (NSCLC). In NSCLC cell lines, we observed lower expression of Testin compared to that noted in normal human bronchial epithelial cells. MTT assays, flow cytometry, clonogenic assay and invasion assay showed that the overexpression of the Testin gene inhibited cancer cell proliferation, invasion and colony formation. In tumor xenograft models, Testin markedly inhibited lung cancer cell xenograft formation and growth in athymic nude mice. Taken together, these data suggest that Testin plays an important role in the development and progression of NSCLC. Testin may be an effective novel target in NSCLC prevention and treatment.