[Genome-wide screening of predicted sugar transporters in Neurospora crassa and the application in hexose fermentation by Saccharomyces cerevisiae].
[Genome-wide screening of predicted sugar transporters in Neurospora crassa and the application in hexose fermentation by Saccharomyces cerevisiae].
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DOI：
10.13345/j.cjb.160290
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发表时间：
2017-01-25
期刊：
影响因子：
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通讯作者：
Tian, Chaoguang
中科院分区：
文献类型：
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作者：
Gao, Jingfang;Wang, Bang;Tian, Chaoguang
木质纤维素分解丝状真菌粗糙脉孢菌能够同化各种单糖和寡糖。然而，基因组中超过一半的预测糖转运蛋白仍在等待功能阐明。在这项研究中，在全基因组水平上对粗糙链孢霉中预测的糖转运蛋白的底物光谱进行了系统分析。 NCU01868和NCU08152具有摄取多种己糖的能力，分别命名为NcHXT-1和NcHXT-2。通过荧光共振能量转移分析进一步证实了它们对葡萄糖的转运活性。在无己糖转运酵母 EBY.VW4000 中过表达 NcHXT-1 或 NcHXT-2 可恢复以葡萄糖、半乳糖或甘露糖作为唯一碳源的深层发酵下的生长和乙醇发酵。在多种纤维素分解真菌中发现了 NcHXT-1/-2 同源物。通过基因组扫描对两种丝状真菌保守的己糖转运蛋白 NcHXT-1/-2 进行功能鉴定，将代表目前在工程纤维素分解真菌和酵母中己糖发酵方面所做的努力的新目标。
The lignocellulolytic filamentous fungus Neurospora crassa is able to assimilate various mono- and oligo-saccharides. However, more than half of predicted sugar transporters in the genome are still waiting for functional elucidation. In this study, system analysis of substrate spectra of predicted sugar transporters in N. crassa was performed at genome-wide level. NCU01868 and NCU08152 have the capability of uptaking various hexose, which are named as NcHXT-1 and NcHXT-2 respectively. Their transport activities for glucose were further confirmed by fluorescence resonance energy transfer analysis. Over-expression of either NcHXT-1 or NcHXT-2 in the null-hexose-transporter yeast EBY.VW4000 restored the growth and ethanol fermentation under submerged fermentation with glucose, galactose, or mannose as the sole carbon source. NcHXT-1/-2 homologues were found in a variety of cellulolytic fungi. Functional identification of two filamentous fungal-conserved hexose transporters NcHXT-1/-2 via genome scanning would represent novel targets for ongoing efforts in engineering cellulolytic fungi and hexose fermentation in yeast.