Quantification of superparamagenetic iron oxide (SPIO)-labeled cells using MRI
Quantification of superparamagenetic iron oxide (SPIO)-labeled cells using MRI
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DOI：
10.1002/jmri.20978
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发表时间：
2007-08-01
影响因子：
4.4
通讯作者：
Soltanian-Zadeh, Hamid
中科院分区：
文献类型：
--
作者：
Rad, Ali M.;Arbab, Ali S.;Soltanian-Zadeh, Hamid
目的：展示使用磁共振成像（MRI）定量超顺磁性氧化铁（SPIO）标记细胞的可行性。材料和方法：用氧化铁-硫酸鱼精蛋白复合物（FE-PRO）标记淋巴细胞和9L大鼠胶质肉瘤细胞。细胞被有效标记（大于95%），并通过分光光度法测量每个细胞内的铁浓度（4.77-30.21 pg），制作含有不同数量标记或未标记细胞以及不同浓度FE-PRO的幻影管。此外，将标记和未标记的细胞注射到新鲜和固定的大鼠大脑中。结果：标记和未标记的细胞的细胞活力和增殖相似。使用 7T 和 3T MRI 系统获取 T2 加权图像，并制作含有细胞、游离 FE-PRO 和大脑的管的 R2 图。 R2 值和标记细胞数之间存在很强的线性相关性，但淋巴细胞和胶质肉瘤细胞的回归线不同。同样，R2 值和游离铁之间也存在很强的相关性。然而，对于相同铁浓度，游离铁的 R2 值高于标记细胞。结论：我们的数据表明，通过仔细考虑不同因素和特定对照组，可以对标记细胞进行体内定量。
Purpose: To show the feasibility of using magnetic resonance imaging (MRI) to quantify superparamagnetic iron oxide (SPIO)-labeled cells.Materials and Methods: Lymphocytes and 9L rat gliosarcoma cells were labeled with ferumoxides-protamine sulfate complex (FE-PRO). The cells were labeled efficiently (more than 95%) and the iron concentration inside each cell was measured by spectrophotometry (4.77-30.21 pg), Phantom tubes containing different numbers of labeled or unlabeled cells, as well as different concentrations of FE-PRO, were made. In addition, labeled and unlabeled cells were injected into fresh and fixed rat brains.Results: Cellular viability and proliferation of labeled and unlabeled cells were shown to be similar. T2-weighted images were acquired using 7T and 3T MRI systems, and R2 maps of the tubes containing cells, free FE-PRO, and brains were made. There was a strong linear correlation between R2 values and labeled cell numbers, but the regression lines were different for the lymphocytes and gliosarcoma cells. Similarly, there was strong correlation between R2 values and free iron. However, free iron had higher R2 values than the labeled cells for the same concentration of iron.Conclusion: Our data indicate that in vivo quantification of labeled cells can be done by careful consideration of different factors and specific control groups.