In vitro antibiofilm and anti-adhesion effects of magnesium oxide nanoparticles against antibiotic resistant bacteria
In vitro antibiofilm and anti-adhesion effects of magnesium oxide nanoparticles against antibiotic resistant bacteria
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DOI：
10.1111/1348-0421.12580
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发表时间：
2018-04-01
影响因子：
2.6
通讯作者：
Jamil, Saba
中科院分区：
文献类型：
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作者：
Hayat, Sumreen;Muzammil, Saima;Jamil, Saba
本研究的目的是确定MgO纳米颗粒（NPs）对耐药细菌临床菌株的抗菌和抗菌膜潜力。通过湿化学方法合成MgO纳米颗粒，并通过扫描电子显微镜和能量色散X射线对其进行进一步表征。采用微量肉汤稀释法和琼脂扩散法测定其抗菌活性。使用Bradford方法评估由于膜完整性丧失导致的细胞蛋白渗漏。采用结晶紫染色后的微量滴定板测定来确定MgO NP对生物膜形成和所建立的生物膜的去除的影响。MIC值在125和500 g/mL之间。此外，用MgO NP处理加速了膜破裂的速率，测量为细胞蛋白质泄漏的函数。革兰氏阴性菌中细胞蛋白含量的渗漏更大。细胞粘附试验表明，25.3-49.8%的细菌附着到塑料表面的抑制。根据静态生物膜法，MgO纳米颗粒以时间依赖性方式将生物膜形成潜力从31%降低到82.9%。此外，纳米颗粒还显著降低了48、72、96和120小时生物膜的生物量（P
The aim of the current investigation was to determine the antibacterial and antibiofilm potential of MgO nanoparticles (NPs) against antibiotic-resistant clinical strains of bacteria. MgO NPs were synthesized by a wet chemical method and further characterized by scanning electron microscopy and energy dispersive X-ray. Antibacterial activity was determined by broth microdilution and agar diffusion methods. The Bradford method was used to assess cellular protein leakage as a result of loss of membrane integrity. Microtiter plate assay following crystal violet staining was employed to determine the effect of MgO NPs on biofilm formation and removal of established biofilms. MIC values ranged between 125 and 500g/mL. Moreover, treatment with MgO NPs accelerated rate of membrane disruption, measured as a function of leakage of cellular proteins. Leakage of cellular protein content was greater among gram-negative bacteria. Cell adherence assay indicated 25.3-49.8% inhibition of bacterial attachment to plastic surfaces. According to a static biofilm method, MgO NPs reduced biofilm formation potential from 31% to 82.9% in a time-dependent manner. Moreover, NPs also significantly reduced the biomass of 48, 72, 96 and 120hr old biofilms (P