Elevated concentrations of sphingosylphosphorylcholine in cerebrospinal fluid after subarachnoid hemorrhage: A possible role as a spasmogen
Elevated concentrations of sphingosylphosphorylcholine in cerebrospinal fluid after subarachnoid hemorrhage: A possible role as a spasmogen
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DOI：
10.1016/j.jocn.2009.01.010
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发表时间：
2009-08-01
影响因子：
2
通讯作者：
Suzuki, Michiyasu
中科院分区：
文献类型：
--
作者：
Kurokawa, Tetsu;Yumiya, Yasunobu;Suzuki, Michiyasu
本研究探讨了鞘酰磷胆碱（SPC）在蛛网膜下腔出血（SAH）后脑血管痉挛机制中的作用。测定了SAH患者脑脊液（CSF）和实验犬模型中SPC的水平。采集SAH患者的脑脊液样本，以及大池注射SPC的狗的脑脊液样本，以检测CSF中SPC的动力学。采用固相萃取和三重四极杆质谱法测定SPC。SAH患者在发病后第3、8、14天SPC浓度明显高于正常脑脊液。在犬模型中，观察到CSF中SPC的快速稀释。结合以往的研究数据，这些结果表明SPC参与了脑血管痉挛的发展。CSF中SPC的快速稀释表明SPC释放到CSF中的浓度高于本研究中测量的浓度。2009爱思唯尔有限公司版权所有。
This study investigates the role of sphingosylphosphorylcholine (SPC) in the mechanisms underlying cerebral vasospasm after subarachnoid hemorrhage (SAH). The levels of SPC were measured in cerebrospinal fluid (CSF) of patients with SAH and also in an experimental canine model. CSF samples were collected from I I patients with SAH, and from dogs that had received an injection of SPC into the cisterna magna to examine SPC kinetics in the CSF. SPC was assayed using solid-phase extraction and triple quadrupole mass spectrometry. The SPC concentrations in SAH patients on days 3, 8, and 14 after the onset of SAH were significantly higher than those in normal CSF In the canine model, rapid dilution of SPC in CSF was observed. In combination with data from previous studies, these results Suggest that SPC is involved in the development of cerebral vasospasm. Rapid dilution of SPC in CSF suggests that SPC is released into CSF at higher concentrations than those measured in the present study. (C) 2009 Elsevier Ltd. All rights reserved.