Dextran hydrogels via disulfide-containing Schiff base formation: Synthesis, stimuli-sensitive degradation and release behaviors
Dextran hydrogels via disulfide-containing Schiff base formation: Synthesis, stimuli-sensitive degradation and release behaviors
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DOI：
10.1016/j.carbpol.2021.118085
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发表时间：
2021-04-30
影响因子：
11.2
通讯作者：
Shan, Shaoyun
中科院分区：
文献类型：
--
作者：
Su, Hongying;Zheng, Rong;Shan, Shaoyun
基于葡聚糖氧化多醛右旋糖苷和半胱胺形成席夫碱键，即含二硫键的席夫碱反应，通过简便的方法合成了同时含有二硫键和席夫碱键的葡聚糖水凝胶。流变性、溶胀性和C-13CP/MAS核磁共振研究结果表明，DEX-SS水凝胶的交联度与-CHO/-NH_2的摩尔比密切相关。用扫描电子显微镜、流变学研究和Ellman实验研究了Dex-SS水凝胶的酸性和还原型(GSH)环境敏感降解行为。此外，阿霉素(DOX)被负载到水凝胶骨架中，并表现出对pH/GSH的响应释放行为。通过与HepG2细胞的Transwell实验，证实了Dex-SS水凝胶的细胞相容性和对释放的DOX的有效摄取。含二硫键的席夫碱反应利用多种多糖上丰富的邻位羟基，被认为是开发局部给药刺激性敏感水凝胶的一种通用方法。
Dextran hydrogels (Dex-SS) containing both disulfide and Schiff base bonds were developed via facile method based on the dextran oxidation and subsequent formation of Schiff base linkages between polyaldehyde dextran and cystamine, denoted as the disulfide-containing Schiff base reactions. Results of rheology, swelling and C-13 CP/MAS NMR study indicated that cross-linking degree of Dex-SS hydrogels depended strongly on the molar ratio of -CHO/-NH2. Acidic and reductive (GSH) environment sensitive degradation behaviors of Dex-SS hydmgels were then evidenced by SEM, rheology study and Ellman's assay. Moreover, doxorubicin (DOX) was loaded into the hydrogel matrix and pH/GSH-responsive release behaviors were demonstrated. Cyto-compatibility of Dex-SS hydrogel and effective cell uptake of released DOX was finally proved by transwell assay with HepG2 cells. Take advantages of the abundance of vicinal hydroxyl on a variety of polysaccharides, the disulfide-containing Schiff base reactions is considered as versatile method to develop stimuli-sensitive hydrogels for local drug delivery.