Mobilization of iron from cellular ferritin by ascorbic acid in neuroblastoma SK-N-SH cells: An EPR study
Mobilization of iron from cellular ferritin by ascorbic acid in neuroblastoma SK-N-SH cells: An EPR study
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DOI：
10.1016/0014-5793(96)00098-1
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发表时间：
1996-02-26
期刊：
影响因子：
3.5
通讯作者：
Matzanke, BF
中科院分区：
文献类型：
--
作者：
Baader, SL;Bill, E;Matzanke, BF
用电子顺磁共振（EPR）谱原位分析了抗坏血酸对细胞内铁蛋白中铁的动员作用。EPR使铁蛋白和其他Fe ~（3+）结合的细胞组分之间的区别成为可能。铁蛋白的有序铁核心产生共振信号，该信号仅在高于50 K的温度下才能观察到。在本研究中，我们清楚地表明，抗坏血酸是能够动员铁从铁蛋白在细胞系统中减少神经母细胞瘤SK-N-SH细胞中的铁离子核心。这一机制可能为治疗这种难以治愈的IV期肿瘤开辟新的途径，特别是与某些细胞抑制药物联合使用。
The mobilization of iron from intracellular ferritin by ascorbic acid has been analysed in situ by electron paramagentic resonance (EPR) spectroscopy. EPR enables a distinction between ferritins and other Fe3+-binding cellular components, The ordered iron core of ferritin gives rise to a resonance signal which can be observed only at temperatures above 50 K. In the present study we clearly demonstrate that ascorbic acid is capable of mobilizing iron from ferritin in the cellular system by reduction of the ferric ion core in neuroblastoma SK-N-SH cells. This mechanism may open new ways in the therapy of this hardly curable tumor in stage IV, especially in combination with some cytostatic drugs.