Development of 32 novel microsatellite loci in Juglans sigillata using genomic data
Development of 32 novel microsatellite loci in Juglans sigillata using genomic data
复制标题
利用基因组数据开发核桃中 32 个新的微卫星位点
DOI：
10.1002/aps3.11328
复制
发表时间：
2020-02
影响因子：
3.6
通讯作者：
Li De-Zhu
中科院分区：
文献类型：
--
作者：
Xu Zu-Chang;Jin Ye-Chuan;Milne Richard I;Xiahou Zuo-Ying;Qin Han-Tao;Ye Lin-Jiang;Gao Lian-Ming;Liu Jie;Li De-Zhu
为了研究核桃及其近缘种的遗传结构和驯化历史，对我国西南地区特有的核桃科核桃（Juglanssigillata）进行了微卫星标记研究。方法和结果利用基因组数据构建了32个微卫星位点，并对3个自然群体的60个个体进行了检测。这些引物检测到高水平的多态性，每个位点观察到多达8个等位基因，平均4个等位基因的人群。观察到的和预期的杂合性水平分别为0.000-1.000和0.000-0.785。除了两个位点外，所有位点也在三个密切相关的欧亚核桃物种（核桃，核桃，核桃和核桃mandshurica）中成功扩增。结论微卫星标记为研究核桃属植物的遗传结构、驯化历史和品种鉴定提供了有力的资源。
Premise A novel set of microsatellite markers was developed for Juglans sigillata (Juglandaceae), an endemic walnut species in southwestern China, to facilitate cultivar identification and future investigations into the genetic structure and domestication history of this species and its close relatives. Methods and Results We developed 32 microsatellite loci for J. sigillata using genomic data and used them to examine 60 individuals from three natural populations. A high level of polymorphism was detected by these primers, with up to eight alleles observed per locus, and an average of four alleles across populations. The levels of observed and expected heterozygosity ranged from 0.000–1.000 and 0.000–0.785, respectively. All but two of the loci were also successfully amplified in three closely related Eurasian Juglans species (J. regia, J. cathayensis, and J. mandshurica). Conclusions The microsatellite loci identified here provide a powerful resource for examining the genetic structure and domestication history of Juglans, as well as identification of its cultivars.