Analytical study of microsomes and isolated subcellular membranes from rat liver. V. Immunological localization of cytochrome b5 by electron microscopy: methodology and application to various subcellular fractions
Analytical study of microsomes and isolated subcellular membranes from rat liver. V. Immunological localization of cytochrome b5 by electron microscopy: methodology and application to various subcellular fractions
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大鼠肝脏微粒体和分离的亚细胞膜的分析研究。
作者：
S. Fowler;J. Remacle;A. Trouet;H. Beaufay;J. Berthet;M. Wibo;P. Hauser
用抗细胞色素b5/抗铁蛋白杂合抗体和铁蛋白标记的细胞免疫学方法，研究了细胞色素b5在大鼠肝脏不同亚细胞细胞器膜上的定位。在这项研究中，使用了高纯度和生化表征的膜制剂。外膜线粒体膜被杂合抗体大量标记，而高尔基体和质膜不被试剂标记。杂合抗体中等程度地标记了过氧化酶体膜，表明它们可能含有一些细胞色素b5。介绍了一种无需消化的杂合抗体的制备和纯化方法，并对最终试剂产品的组成进行了分析。
The localization of cytochrome b5 on the membranes of various subcellular organelles of rat liver was studied by a cytoimmunological procedure using anti-cytochrome b5/anti-ferritin hybrid antibodies and ferritin as label. For this study, highly purified and biochemically characterized membrane preparations were employed. Outer mitochondrial membranes were found to be heavily labeled by the hybrid antibodies whereas Golgi and plasma membranes were not marked by the reagent. Peroxisome membranes were moderately labeled by the hybrid antibodies, suggesting that they may contain some cytochrome b5. The preparation and purification of hybrid antibodies without peptic digestion is described and an analysis made of the composition of the final reagent product.