RINGER'S MALATE SOLUTION PROTECTS AGAINST THE MULTIPLE ORGAN INJURY AND DYSFUNCTION CAUSED BY HEMORRHAGIC SHOCK IN RATS
RINGER'S MALATE SOLUTION PROTECTS AGAINST THE MULTIPLE ORGAN INJURY AND DYSFUNCTION CAUSED BY HEMORRHAGIC SHOCK IN RATS
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DOI：
10.1097/shk.0b013e318264e664
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发表时间：
2012-09-01
期刊：
影响因子：
3.1
通讯作者：
Yao, Shang-Long
中科院分区：
文献类型：
--
作者：
Dai, Zhong-Liang;Wu, Jing;Yao, Shang-Long
苹果酸，以其阴离子苹果酸盐的形式，是被称为柠檬酸或克雷布斯循环的主要生物化学能量产生循环中的关键中间体。在这项研究中，作者使用大鼠模型研究了一种新型苹果酸林格氏晶体溶液在失血性休克液体复苏后的保护作用。在全身麻醉下，对Sprague-Dawley雄性大鼠进行60分钟的失血性休克（40 mmHg持续60分钟），然后进行晶体复苏。分组如下：（1）假休克，（2）生理盐水，（3）乳酸林格氏液，和（4）苹果酸林格氏液。复苏后4 h终止实验。在实验过程中监测平均动脉血压（MAP）和血液生物物理参数。检测血浆丙氨酸氨基转移酶、天冬氨酸氨基转移酶、尿素、肌酐、超氧化物歧化酶和丙二醛水平。对肠、肝、肺和肾进行组织病理学检查。结果表明，苹果酸能迅速增加MAP，并能维持MAP较长时间。林格氏苹果酸可降低丙氨酸氨基转移酶、天冬氨酸氨基转移酶、尿素和肌酐。同时，提高了超氧化物歧化酶的活性，降低了丙二醛的含量。组织病理学表明，林格氏苹果酸对大鼠失血性休克引起的多器官损伤有保护作用。林格氏苹果酸预防失血性休克动物循环衰竭和减轻多器官功能障碍综合征。这项研究表明，林格氏苹果酸溶液可能是一种潜在的新型液体复苏治疗剂。
Malic acid, in the form of its anion malate, is a key intermediate in the major biochemical energy-producing cycle known as the citric acid or Krebs cycle. In this study, the authors investigated the protective effect of a novel crystalloid solution of Ringer's malate following fluid resuscitation of hemorrhagic shock using a rat model. Under general anesthesia, Sprague-Dawley male rats were subjected to 60 min of hemorrhagic shock (40 mmHg for 60 min) followed by crystalloid resuscitation. Groups were as follows: (1) sham shock, (2) normal saline, (3) Ringer's lactate, and (4) Ringer's malate. The experiment was terminated at 4 h after resuscitation. Mean arterial blood pressure (MAP) and blood biophysical parameters were monitored during the experiment. The alanine aminotransferase, aspartate aminotransferase, urea, creatinine, superoxide dismutase, and malondialdehyde levels in plasma were detected. The intestine, liver, lung, and renal histopathology were measured. It was found that Ringer's malate could increase MAP immediately and maintain MAP for a long time. Ringer's malate could reduce the level of alanine aminotransferase, aspartate aminotransferase, urea, and creatinine. At the same time, the activity of superoxide dismutase was increased, and the level of malondialdehyde was decreased. Histopathology indicated that Ringer's malate can protect against the multiple organ injury caused by hemorrhagic shock in rats. Ringer's malate prevented circulatory failure and alleviated multiple organ dysfunction syndrome in animals with hemorrhagic shock. The study suggests that Ringer's malate solution could be a potential novel therapeutic agent for fluid resuscitation.