THE COMPLETE DNA-SEQUENCE OF VACCINIA VIRUS
THE COMPLETE DNA-SEQUENCE OF VACCINIA VIRUS
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DOI：
10.1016/0042-6822(90)90294-2
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发表时间：
1990-11-01
期刊：
影响因子：
3.7
通讯作者：
PAOLETTI, E
中科院分区：
文献类型：
--
作者：
GOEBEL, SJ;JOHNSON, GP;PAOLETTI, E
牛痘病毒基因组的完整DNA序列已经确定。基因组全长191，636 bp，碱基组成为66.6% A + T。我们已经确定了198个“主要”蛋白质编码区和65个重叠的“次要”区域，共263个潜在的基因。通过检查DNA序列特征并与现有的牛痘病毒作图分析、序列数据和转录数据进行比较，定位了病毒编码的基因。发现这些基因在基因组中沿着排列，非编码序列区域相对较少。尽管识别出了与已知功能的蛋白质的几个相似性，但由这些开放阅读框编码的大多数蛋白质的功能尚未确定。
The complete DNA sequence of the genome of vaccinia virus has been determined. The genome consisted of 191,636 bp with a base composition of 66.6% A + T. We have identified 198 "major" protein-coding regions and 65 overlapping "minor" regions, for a total of 263 potential genes. Genes encoded by the virus were located by examination of DNA sequence characteristics and compared with existing vaccinia virus mapping analyses, sequence data, and transcription data. These genes were found to be compactly organized along the genome with relatively few regions of noncoding sequences. Whereas several similarities to proteins of known function were discerned, the function of the majority of proteins encoded by these open reading frames is as yet undetermined.