Oligosaccharides from human milk.
Oligosaccharides from human milk.
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DOI：
10.1016/0076-6879(78)50023-2
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发表时间：
1978-01-01
影响因子：
--
通讯作者：
Tachibana, Y
中科院分区：
文献类型：
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作者：
Kobata, A;Yamashita, K;Tachibana, Y
本文综述了自早期报道[酶学方法28，24]以来开发的用于分离乳低聚糖的技术以及新发现的糖的结构。在凝胶渗透色谱中，Bio-Gel P-4柱比Sephadex G-25柱具有上级的性能，硼酸盐纸电泳可用于从同分异构体寡糖混合物中分离具有Fuc α 1“右箭头”2Gal基团的寡糖。描述了从人乳中分离以下寡糖：6 ′-半乳糖基乳糖;乳糖-N-岩藻五糖;二岩藻糖基-β-乳糖-N-己糖和二岩藻糖基-β-乳糖-N-新己糖;以及岩藻糖基乳糖-N-八糖和岩藻糖基乳糖-N-新八糖。
Techniques developed for isolating milk oligosaccharides since the earlier report [Methods in Enzymology 28, 24] and structures of newly found sugars, are reviewed. Bio-Gel P-4 columns have been found superior to Sephadex G-25 columns in gel permeation chromatography, and borate paper electrophoresis has been found useful for the separation of oligosaccharides with Fuc alpha 1 'right arrow' 2Gal grouping from isomeric oligosaccharide mixtures. Isolation of the following oligosaccharides from human milk is described: 6'-galactosyllactose; lacto-N-fucopentaose; difucosyl-p-lacto-N-hexaose and difucosyl-p-lacto-N-neohexaose; and fucosyllacto-N-octaose and fucosyllacto-N-neooctaose.